Contrasting macrophage activation by fine and ultrafine titanium dioxide particles is associated with different uptake mechanisms

Autor: Christine R. Rose, Frederik-Jan van Schooten, Damien van Berlo, Julia Langer, Petra Haberzettl, Roel P. F. Schins, Agnes M. Scherbart, Alexey Bushmelev, Catrin Albrecht, Annette M. Schmidt
Přispěvatelé: RS: NUTRIM - R4 - Gene-environment interaction, Farmacologie en Toxicologie
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Health
Toxicology and Mutagenesis
LUNG EPITHELIAL-CELLS
Interleukin-1beta
NF-KAPPA-B
Gene Expression
Nitric Oxide Synthase Type II
Toxicology
chemistry.chemical_compound
size distribution
OXIDATIVE STRESS
ALVEOLAR MACROPHAGES
particle internalization
Cytochalasin D
Titanium
Air Pollutants
agglomeration
biology
Pinocytosis
General Medicine
NALP3 INFLAMMASOME
Silicon Dioxide
Endocytosis
Nitric oxide synthase
TRANSCRIPTION FACTORS
Biochemistry
Intracellular
Materials science
Cell Survival
Chlorpromazine
Phagocytosis
lcsh:Industrial hygiene. Industrial welfare
SURFACE-AREA
Cell Line
ENVIRONMENTAL PARTICLES
lcsh:RA1190-1270
PULMONARY INFLAMMATION
Macrophages
Alveolar
Extracellular
Animals
Particle Size
lcsh:Toxicology. Poisons
Tumor Necrosis Factor-alpha
titanium dioxide
Research
Receptors
IgG
Macrophage Activation
Rats
Heme oxygenase
NR8383 cells
chemistry
SCAVENGER RECEPTOR MARCO
Heme Oxygenase (Decyclizing)
Biophysics
biology.protein
Nanoparticles
Calcium
Reactive Oxygen Species
lcsh:HD7260-7780.8
Zdroj: Particle and Fibre Toxicology, 8(1):31. BioMed Central Ltd
Particle and Fibre Toxicology, Vol 8, Iss 1, p 31 (2011)
Particle and Fibre Toxicology
ISSN: 1743-8977
Popis: Inhalation of (nano)particles may lead to pulmonary inflammation. However, the precise mechanisms of particle uptake and generation of inflammatory mediators by alveolar macrophages (AM) are still poorly understood. The aim of this study was to investigate the interactions between particles and AM and their associated pro-inflammatory effects in relation to particle size and physico-chemical properties. NR8383 rat lung AM were treated with ultrafine (uf), fine (f) TiO2 or fine crystalline silica (DQ12 quartz). Physico-chemical particle properties were investigated by transmission electron microscopy, elemental analysis and thermogravimetry. Aggregation and agglomeration tendency of the particles were determined in assay-specific suspensions by means of dynamic light scattering. All three particle types were rapidly taken up by AM. DQ12 and ufTiO2 , but not fTiO2 , caused increased extracellular reactive oxygen species (ROS), heme oxygenase 1 (HO-1) mRNA expression and tumor necrosis factor (TNF)-α release. Inducible nitric oxide synthase (iNOS) mRNA expression was increased most strongly by ufTiO2 , while DQ12 exclusively triggered interleukin (IL) 1β release. However, oscillations of intracellular calcium concentration and increased intracellular ROS were observed with all three samples. Uptake inhibition experiments with cytochalasin D, chlorpromazine and a Fcγ receptor II (FcγRII) antibody revealed that the endocytosis of fTiO2 by the macrophages involves actin-dependent phagocytosis and macropinocytosis as well as clathrin-coated pit formation, whereas the uptake of ufTiO2 was dominated by FcγIIR. The uptake of DQ12 was found to be significantly reduced by all three inhibitors. Our findings suggest that the contrasting AM responses to fTiO2 , ufTiO2 and DQ12 relate to differences in the involvement of specific uptake mechanisms.
Databáze: OpenAIRE
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