Developmental and comparative transcriptomic identification of iridophore contribution to white barring in clownfish
| Autor: | Laurence Besseau, Carine Rey, Marie-Line Escande, Marie Sémon, David M. Parichy, Natacha Roux, Vincent Laudet, Thibault Lorin, Jean-Nicolas Volff, Pauline Salis, Nicolas Salamin, Victor M Lewis, Anna Marcionetti |
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| Přispěvatelé: | Biologie intégrative des organismes marins (BIOM), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lausanne = University of Lausanne (UNIL), Centre de recherches insulaires et observatoire de l'environnement (CRIOBE), Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Department of Biology, Department of Cell Biology, University of Virginia, Charlottesville, Virgi, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Biology, University of Washington, Seattle, Washington, Swiss Institute of Bioinformatics [Lausanne] (SIB), Okinawa Institute of Science and Technology, Okinawa Institute of Science and Technology Graduate University (OIST) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fish Proteins Cell type genetic structures leucophore [SDV]Life Sciences [q-bio] Dermatology Biology iridophore Genome General Biochemistry Genetics and Molecular Biology Article Transcriptome 03 medical and health sciences Pigment 0302 clinical medicine Amphiprion Animals Chromatophores Gene Zebrafish coral reef fish Pigmentation Fishes clownfish Gene Expression Regulation Developmental transcriptomic biology.organism_classification Chromatophore White (mutation) 030104 developmental biology Oncology Evolutionary biology 030220 oncology & carcinogenesis visual_art visual_art.visual_art_medium |
| Zdroj: | Pigment Cell & melanoma research Pigment Cell & melanoma research, 2019, 32 (3), pp.391-402. ⟨10.1111/pcmr.12766⟩ Pigment Cell Melanoma Res |
| DOI: | 10.1111/pcmr.12766⟩ |
| Popis: | International audience; Actinopterygian fishes harbor at least eight distinct pigment cell types, leading to a fascinating diversity of colors. Among this diversity, the cellular origin of the white color appears to be linked to several pigment cell types such as iridophores or leuco‐phores. We used the clownfish Amphiprion ocellaris, which has a color pattern con‐sisting of white bars over a darker body, to characterize the pigment cells that underlie the white hue. We observe by electron microscopy that cells in white bars are similar to iridophores. In addition, the transcriptomic signature of clownfish white bars ex‐hibits similarities with that of zebrafish iridophores. We further show by pharmaco‐logical treatments that these cells are necessary for the white color. Among the top differentially expressed genes in white skin, we identified several genes (fhl2a, fhl2b, saiyan, gpnmb, and apoD1a) and show that three of them are expressed in iridophores. Finally, we show by CRISPR/Cas9 mutagenesis that these genes are critical for irido‐phore development in zebrafish. Our analyses provide clues to the genomic under‐pinning of color diversity and allow identification of new iridophore genes in fish |
| Databáze: | OpenAIRE |
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