N-acetylcysteine attenuates lipopolysaccharide-induced impairment in lamination of Ctip2-and Tbr1- expressing cortical neurons in the developing rat fetal brain
| Autor: | Yu-Hsiu Yang, Chia-Yi Tseng, Tzu-Yun Chu, Yu-Chen Chuang, Chie-Pein Chen, Ming-Wei Chao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male medicine.medical_specialty Lipopolysaccharide Nerve Tissue Proteins medicine.disease_cause Article Acetylcysteine Fetal Development 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cortex (anatomy) Internal medicine medicine Animals Humans Inflammation Neurons Fetus Multidisciplinary Neocortex biology Tumor Suppressor Proteins Brain Gene Expression Regulation Developmental Anatomy Matrix Attachment Region Binding Proteins Rats Repressor Proteins Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry biology.protein TBR1 Neuron T-Box Domain Proteins 030217 neurology & neurosurgery Oxidative stress medicine.drug Transcription Factors |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep32373 |
| Popis: | Oxidative stress and inflammatory insults are the major instigating events of bacterial intrauterine infection that lead to fetal brain injury. The purpose of this study is to investigate the remedial effects of N-acetyl-cysteine (NAC) for inflammation-caused deficits in brain development. We found that lipopolysaccharide (LPS) induced reactive oxygen species (ROS) production by RAW264.7 cells. Macrophage-conditioned medium caused noticeable cortical cell damage, specifically in cortical neurons. LPS at 25 μg/kg caused more than 75% fetal loss in rats. An increase in fetal cortical thickness was noted in the LPS-treated group. In the enlarged fetal cortex, laminar positioning of the early born cortical cells expressing Tbr1 and Ctip2 was disrupted, with a scattered distribution. The effect was similar, but minor, in later born Satb2-expressing cortical cells. NAC protected against LPS-induced neuron toxicity in vitro and counteracted pregnancy loss and alterations in thickness and lamination of the neocortex in vivo. Fetal loss and abnormal fetal brain development were due to LPS-induced ROS production. NAC is an effective protective agent against LPS-induced damage. This finding highlights the key therapeutic impact of NAC in LPS-caused abnormal neuronal laminar distribution during brain development. |
| Databáze: | OpenAIRE |
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