Methods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography
| Autor: | C. C. Sweeley, R. C. Gaver |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Chromatography
Sphingolipids Chromatography Gas Sphingosine Trimethylsilyl Research General Chemical Engineering Organic Chemistry Hydrochloric acid Lipids Sphingolipid Chemistry Techniques Analytical chemistry.chemical_compound chemistry Reagent Organic chemistry lipids (amino acids peptides and proteins) Gas chromatography Hydrogen chloride Sphingomyelin Methane |
| Zdroj: | Journal of the American Oil Chemists' Society. 42:294-298 |
| ISSN: | 0003-021X |
| DOI: | 10.1007/bf02540132 |
| Popis: | A new, simple procedure is described for the direct gas chromatographic determination of sphingolipid bases as their trimethylsilyl derivatives. This method is used to study the nature of the mixture of bases produced by acidic methanolysis of ceramides, sphingomyelins, free sphingolipid bases, andN-acetyl derivatives of the latter. Cleavage of sphingolipids with anhydrous methanolic hydrogen chloride results in the production of considerable quantities of secondary products from sphingosine, such as theO-methyl ethers, which are not formed during methanolysis of free sphingosine. A modified reagent for methanolysis, containing methanol, water and hydrochloric acid, reduces the yield of these by-products to low levels. Preliminary purification of the sphingolipid bases, before gas chromatography, is achieved by chromatographic procedures. Evidence is presented for the occurrence of C16-sphingosine in sphingomyelin from human blood plasma. |
| Databáze: | OpenAIRE |
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