Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix
| Autor: | Josef Jančář, Daniel Tvrdík, Jana Juhasova, Jan Klepacek, Š. Melčáková, Zbyněk Tonar, Lucy Vojtová, Martin Plencner, Evžen Amler, Michala Rampichová, R. Jakubová, Petra Kochová, Alois Nečas, Eva Prosecká |
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| Rok vydání: | 2011 |
| Předmět: |
Scaffold
Materials science Biomedical Engineering Biocompatible Materials Matrix (biology) Bone tissue Biomaterials Elastic Modulus Materials Testing Cell Adhesion medicine Animals Humans Composite scaffold Cell Proliferation Osteoblasts Tissue Scaffolds Cell growth Mesenchymal stem cell Metals and Alloys Cell Differentiation Mesenchymal Stem Cells In vitro Extracellular Matrix Cell biology medicine.anatomical_structure Ceramics and Composites Cattle Collagen Hydroxyapatites Stem cell Porosity Biomarkers Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. :307-315 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.33189 |
| Popis: | Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution. |
| Databáze: | OpenAIRE |
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