Ampicillin biotransformation by a nitrifying consortium
| Autor: | Flor de María Cuervo López, Anne-Claire Texier, José Muñoz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Physiology 01 natural sciences Applied Microbiology and Biotechnology Mineralization (biology) 03 medical and health sciences Bioreactors Biotransformation 010608 biotechnology Ampicillin Ammonium Compounds medicine Food science Effluent 0303 health sciences biology Bacteria 030306 microbiology Chemistry Biosorption General Medicine biology.organism_classification Nitrification Biodegradation Environmental Nitrifying bacteria Batch Cell Culture Techniques Sewage treatment Biotechnology medicine.drug |
| Zdroj: | World journal of microbiologybiotechnology. 36(2) |
| ISSN: | 1573-0972 |
| Popis: | Ampicillin is a widely used β-lactam antibiotic that has been detected in various effluents and can alter biological processes used in wastewater treatment such as nitrification. Physiological and kinetic behaviour of a nitrifying consortium in the presence of ampicillin (AMP) (10, 25, and 50 mg/L) was evaluated in batch cultures. Under the experimental conditions (320 ± 8 mg bacterial protein/L, C/N = 2.4, 24 h), the nitrifying behaviour was very similar among the controls without AMP and the assays with antibiotic, as there was no AMP effect on efficiency (ENH4+ = 99.7 ± 4.2%), yields (YNO2− = 0, YNO3− = 1.0 ± 0.1 mg N/mg NH4+-N consumed), neither specific rates of NH4+ oxidation and NO3− formation. Therefore, nitrifying bacteria were insensitive to AMP presence. At all assayed concentrations, after 24 h, 70.5 ± 3.7% of AMP was removed from the cultures through abiotic (16.0–16.5%), biosorption (23.2–47.0%), and biotransformation (10.0–29.8%) processes. With the increase in the initial AMP concentration, a greater participation of the biotransformation process, associated to an increase in the specific AMP consumption rate was attained. The sludge was able to completely oxidize NH4+ to NO3− by nitrification and eliminate AMP biologically, but without reaching its full mineralization. |
| Databáze: | OpenAIRE |
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