Increased responsiveness of rat mast cells to compound 48/80 due to removal of extracellular magnesium Effects of ouabain and EGTA
| Autor: | Torben Johansen, Henrik Bertelsen |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Male
medicine.medical_specialty Biophysics chemistry.chemical_element Calcium In Vitro Techniques Biochemistry Histamine Release Ouabain Divalent Mast cell chemistry.chemical_compound Structural Biology Internal medicine Genetics medicine Extracellular Animals p-Methoxy-N-methylphenethylamine Magnesium Mast Cells Molecular Biology Egtazic Acid Secretion chemistry.chemical_classification Rats Inbred Strains Cell Biology EGTA Compound 48/80 Rats Kinetics Endocrinology chemistry Histamine medicine.drug |
| Zdroj: | FEBS Letters. (2):185-188 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(91)80584-P |
| Popis: | A decreased secretory response of mast cells to compound 40/80 (12% of control value) after preincubation of the cells with magnesium but without calcium was partially restored by removal of magnesium. EGTA (10 μM) blocked the restoration and decreased the restored secretory activity again, while this was further increased by ouabain (1 mM). Furthermore, ouabain completely restored the decreased secretion (50% of control value) due to preincubation without the divalent cations. This may indicate that magnesium influences a pool of cellular calcium that is involved in the stimulus-secretion coupling and is available to EGTA, and ouabain did not counteract the inhibitory mechanism or magnesium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|