Effects of doxorubicin and docetaxel on susceptibility to apoptosis in high expression level of survivin in HEK and HEK-S cell lines as in vitro models
| Autor: | Kayhan Mehdizadeh, Farangis Ataei, Saman Hosseinkhani |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Survivin Cell Biophysics Caspase 3 Apoptosis Docetaxel Biochemistry Models Biological Inhibitor of Apoptosis Proteins 03 medical and health sciences 0302 clinical medicine Adenosine Triphosphate Cell Movement medicine Humans Doxorubicin neoplasms Molecular Biology Caspase 7 Chemistry HEK 293 cells Cell Biology 030104 developmental biology medicine.anatomical_structure HEK293 Cells Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer cell Cancer research Reactive Oxygen Species medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 532(1) |
| ISSN: | 1090-2104 |
| Popis: | A major problem in the cancer treatment is the inherent resistance to chemotherapy. Identifying proteins that, once introduced in cancer cells, lead to a decreased efficiency of treatment outcome constitutes a major goal for biomedical research and applications. Survivin is a protein of IAPs family which its high expression can be a potential candidate for regulating cell death and survival in cancer therapy. To investigate the association of survivin increment and resistance to drug, survivin-reconstituted HEK (HEK-S) and HEK cells were used as in vitro models for the doxorubicin and docetaxel cellular response. Both morphological observation and survival assay exhibited that survivin reconstitution cells were significantly resistant to apoptotic stimuli by both drugs. It was observed that survivin overexpression has led to a decrease in caspase 3/7 activity and ROS level of cells but an increase in ATP content. Also, survivin-reconstituted cell displayed less red fluorescence compared to control after stimulation by drugs. Moreover, wound healing assay showed the ability of survivin to cause neighbouring cells to increase resistance to induction. These findings demonstrated survivin could be a potential target that can be inhibited the function of different drugs with various mechanisms in chemotherapy. |
| Databáze: | OpenAIRE |
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