Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment
| Autor: | María Questa, Santiago Gabriel Miriuka, María Elida Scassa, Carolina Jaquenod De Giusti, Gabriela Pretre, Guillermo Agustín Videla Richardson, Ricardo Martín Gómez, Carolina Bluguermann, María Florencia Ferrer, Leonardo Romorini, Gustavo Sevlever |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Coxsackievirus Infections
Embryoid body human embryonic stem cell Biology Coxsackievirus Virus Replication Cell Line derived contractile embryoid bodies Interferon medicine Humans Viability assay Embryoid Bodies Embryonic Stem Cells Ciencias Exactas Infectivity Medicine(all) Coxsackievirus B infection Interferon-beta General Medicine Cell Biology biology.organism_classification Embryonic stem cell Molecular biology Enterovirus B Human Viral replication Cell culture Ciencias Médicas Receptors Virus medicine.drug Developmental Biology |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | We studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors were detected in all cell lines and differentiation stages studied. Real-time PCR analysis showed that CAR mRNA levels were 3.4-fold higher in undifferentiated cells, while DAF transcript levels were 2.78-fold more abundant in differentiated cultures (P5-106 plaque forming units (PFU)/ml, the highest titers were detected in undifferentiated cells. Cell viability detected by a colorimetric assay, showed inverse correlation with infectivity titers of cell culture supernatants. Treatment with 100 U of interferon Iβ significantly reduced viral replication and associated cell death during a 24-48 h observation period, as detected by reduced infectivity titers in the supernatants and increased cell viability by a colorimetric assay, respectively. We propose human embryonic stem cell and derived contractile embryoid bodies as a valid model to study cardiac Coxsackievirus B infection. Instituto de Biotecnologia y Biologia Molecular Facultad de Ciencias Exactas |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|