Aloe Metabolites Prevent LPS-Induced Sepsis and Inflammatory Response by Inhibiting Mitogen-Activated Protein Kinase Activation
| Autor: | Shiuan-Pey Lin, Chung Ping Yu, Yu-Chi Hou, Shih-Hua Fang, Chia-Yang Li, Yung Li Hung, Katsuhiko Suzuki, Meng Syuan Yang |
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| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Nitric Oxide Synthase Type II Anthraquinones Pharmacology Nitric Oxide Aloe vera Proinflammatory cytokine Sepsis Mice 03 medical and health sciences Glucuronides 0302 clinical medicine Western blot In vivo medicine Animals Molecular Targeted Therapy Aloe Intestinal Mucosa biology medicine.diagnostic_test Polyphenols General Medicine biology.organism_classification medicine.disease In vitro Rats Plant Leaves RAW 264.7 Cells 030104 developmental biology Liver Complementary and alternative medicine Biochemistry 030220 oncology & carcinogenesis Mitogen-activated protein kinase Macrophages Peritoneal biology.protein Cytokines Inflammation Mediators Mitogen-Activated Protein Kinases Ex vivo Phytotherapy |
| Zdroj: | The American Journal of Chinese Medicine. 45:847-861 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x17500458 |
| Popis: | Aloe, a polyphenolic anthranoid-containing Aloe vera leaves, is a Chinese medicine and a popular dietary supplement worldwide. In in vivo situations, polyphenolic anthranoids are extensively broken down into glucuronides and sulfate metabolites by the gut and the liver. The anti-inflammatory potential of aloe metabolites has not been examined. The aim of this study was to investigate the anti-inflammatory effects of aloe metabolites from in vitro (lipopolysaccharides (LPS)-activated RAW264.7 macrophages) and ex vivo (LPS-activated peritoneal macrophages) to in vivo (LPS-induced septic mice). The production of proinflammatory cytokines (TNF-[Formula: see text] and IL-12) and NO was determined by ELISA and Griess reagents, respectively. The expression levels of iNOS and MAPKs were analyzed by Western blot. Our results showed that aloe metabolites inhibited the expression of iNOS, decreased the production of TNF-[Formula: see text], IL-12, and NO, and suppressed the phosphorylation of MAPKs by LPS-activated RAW264.7 macrophages. In addition, aloe metabolites reduced the production of NO, TNF-[Formula: see text] and IL-12 by murine peritoneal macrophages. Furthermore, aloe administration significantly reduced the NO level and exhibited protective effects against sepsis-related death in LPS-induced septic mice. These results suggest that aloe metabolites exerted anti-inflammatory effects in vivo, and that these effects were associated with the inhibition of inflammatory mediators. Therefore, aloe could be considered an effective therapeutic agent for the treatment of sepsis. |
| Databáze: | OpenAIRE |
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