Differential DNA methylation in experienced meditators after an intensive day of mindfulness-based practice: Implications for immune-related pathways

Autor: Maud Fagny, Antoine Lutz, Raphaëlle Chaix, Marta Cosín-Tomás, Laure Lemée, Béatrice Regnault, Richard J. Davidson, María Jesús Álvarez-López, Perla Kaliman
Přispěvatelé: Eco-Anthropologie et Ethnobiologie (EAE), Muséum national d'Histoire naturelle (MNHN)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Department of Human Genetics [Montréal], McGill University = Université McGill [Montréal, Canada], Departament de Bioquimica i Biologia Molecular and Institut de Biomedicina (IBUB), Universitat de Barcelona (UB), Génotypage des Eucaryotes (Plate-Forme), Institut Pasteur [Paris], Biomics (plateforme technologique), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Center for Healthy Minds [Wisconsin, MA, USA], University of Wisconsin-Madison, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Faculty of Health Sciences [Barcelona, Spain], Universitat Oberta de Catalunya [Barcelona] (UOC), Chaix, Raphaëlle, Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN), Éco-Anthropologie (EA), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Muséum national d'Histoire naturelle (MNHN), McGill University, Plateforme technologique Biomics
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Stress management
Mindfulness
meditation
[SDV]Life Sciences [q-bio]
media_common.quotation_subject
Immunology
[SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics
Methylation Site
Bioinformatics
Article
[SHS]Humanities and Social Sciences
Epigenome
03 medical and health sciences
Behavioral Neuroscience
0302 clinical medicine
Humans
Medicine
Longitudinal Studies
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Meditation
Epigenetics
media_common
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
epigenetics
Endocrine and Autonomic Systems
business.industry
Methylation
DNA Methylation
Middle Aged
peripheral blood mononuclear cells
[SDV] Life Sciences [q-bio]
030104 developmental biology
[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics
[SCCO.PSYC] Cognitive science/Psychology
[SCCO.PSYC]Cognitive science/Psychology
DNA methylation
Leukocytes
Mononuclear
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
[SHS] Humanities and Social Sciences
methylation
business
Stress
Psychological
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
030217 neurology & neurosurgery
Zdroj: Brain, Behavior, and Immunity
Brain, Behavior, and Immunity, Elsevier, 2019, pp.30879-7. ⟨10.1016/j.bbi.2019.11.003⟩
Brain, Behavior, and Immunity, 2019, pp.30879-7. ⟨10.1016/j.bbi.2019.11.003⟩
Brain Behav Immun
ISSN: 0889-1591
1090-2139
DOI: 10.1016/j.bbi.2019.11.003
Popis: International audience; The human methylome is dynamically influenced by psychological stress. However, its responsiveness to stress management remains underexplored. Meditation practice has been shown to significantly reduce stress level, among other beneficial neurophysiological outcomes. Here, we evaluated the impact of a day of intensive meditation practice (t2-t1 = 8 h) on the methylome of peripheral blood mononuclear cells in experienced meditators (n = 17). In parallel, we assessed the influence of a day of leisure activities in the same environment on the methylome of matched control subjects with no meditation experience (n = 17). DNA methylation profiles were analyzed using the Illumina 450 K beadchip array. We fitted for each methylation site a linear model for multi-level experiments which adjusts the variation between t1 and t2 for baseline differences. No significant baseline differences in methylation profiles was detected between groups. In the meditation group, we identified 61 differentially methylated sites (DMS) after the intervention. These DMS were enriched in genes mostly associated with immune cell metabolism and ageing and in binding sites for several transcription factors involved in immune response and inflammation, among other functions. In the control group, no significant change in methylation level was observed after the day of leisure activities. These results suggest that a short meditation intervention in trained subjects may rapidly influence the epigenome at sites of potential relevance for immune function and provide a better understanding of the dynamics of the human methylome over short time windows.
Databáze: OpenAIRE
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