Epidermal Growth Factor Binding and Action on Tympanic Membranes
| Autor: | Nolph Mb, T G O'Daniel, Zogg J, S C Jones, Gregory S. Schultz, Petitjean M, Martinez Sa |
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| Rok vydání: | 1990 |
| Předmět: |
Pathology
medicine.medical_specialty Tympanic Membrane Stromal cell Swine Wounds Penetrating Binding Competitive 03 medical and health sciences 0302 clinical medicine Epidermal growth factor Cornea Epidermal growth factor binding medicine Animals 030223 otorhinolaryngology Receptor Wound Healing Epidermal Growth Factor business.industry Histology General Medicine Hyperplasia medicine.disease Molecular biology ErbB Receptors medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Cats Autoradiography Wound healing business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Annals of Otology, Rhinology & Laryngology. 99:80-84 |
| ISSN: | 1943-572X 0003-4894 |
| Popis: | A significant percentage of tympanic membrane (TM) perforations require some form of therapy to heal. Topical application of epidermal growth factor (EGF), a potent mitogen for epidermal and mesodermal cells, promotes healing of injuries in tissues histologically similar to TM, such as cornea and skin. We evaluated TM as a target tissue for EGF action. Specific, high affinity receptors for EGF were detected in TM (Kd = 3.1 nM, 150 fmol receptor/mg protein). Autoradiography of iodine 125-EGF binding to intact TMs revealed that EGF receptors were present on cells in the stratified squamous epithelial layer and in the stromal/mucoepithelial layer. Repetitive treatment of perforated cat TMs with 20 micrograms of EGF formulated in saline induced substantial hyperplasia of epithelial and stromal layers compared to paired TMs treated with saline. A single treatment of perforated cat TMs with 50 micrograms of EGF formulated in a hydrogel or in shredded Gelfoam produced significantly (p less than .05) smaller perforations at 6 days following the operation compared to paired TMs treated with vehicles. These results demonstrate that TM is a target tissue for EGF and that topical treatment with EGF stimulates healing of TM perforations. |
| Databáze: | OpenAIRE |
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