Effects of hyperbaric oxygen on Pseudomonas aeruginosa susceptibility to imipenem and macrophages
| Autor: | Edlaine Linares, Marcelo Lancellotti, Ohara Augusto, Luciana Maria de Hollanda, Bruna de Araujo Lima, Marcelo Brocchi, Paulo Pinto Joazeiro, Flavia Luna Lima, Selma Giorgio |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
MACRÓFAGOS Imipenem Carbapenem medicine.drug_class Antibiotics Oxidative phosphorylation medicine.disease_cause Microbiology Mice chemistry.chemical_compound Superoxides medicine Animals Cells Cultured chemistry.chemical_classification Hyperbaric Oxygenation Reactive oxygen species biology Pseudomonas aeruginosa Superoxide business.industry Macrophages Drug Synergism biology.organism_classification Anti-Bacterial Agents chemistry Reactive Oxygen Species business Bacteria medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb.14.111 |
| Popis: | ABSTRACT Background: The seriousness to treat burn wounds infected with Pseudomonas aeruginosa led us to examine whether the effect of the carbapenem antibiotic imipenem is enhanced by hyperbaric oxygen (HBO). Materials & methods: The effects of HBO (100% O2, 3 ATA, 5 h) in combination with imipenen on bacterial counts of six isolates of P. aeruginosa and bacterial ultrastructure were investigated. Infected macrophages were exposed to HBO (100% O2, 3 ATA, 90 min) and the production of reactive oxygen species monitored. Results: HBO enhanced the effects of imipenen. HBO increased superoxide anion production by macrophages and likely kills bacteria by oxidative mechanisms. Conclusion: HBO in combination with imipenem can be used to kill P. aeruginosa in vitro and such treatment may be beneficial for the patients with injuries containing the P. aeruginosa. |
| Databáze: | OpenAIRE |
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