The CAF-1 and Hir Histone Chaperones Associate with Sites of Meiotic Double-Strand Breaks in Budding Yeast
| Autor: | Maria-Elisabetta Serrentino, Valérie Borde, Claire Béneut, Elsa Brachet |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
DNA repair
lcsh:Medicine Biology Genetic recombination Fungal Proteins Histones Histone H3 Proliferating Cell Nuclear Antigen Nucleosome DNA Breaks Double-Stranded Histone Chaperones lcsh:Science CAF-1 Genetics Multidisciplinary Microbial Viability lcsh:R fungi Spores Fungal Chromatin Nucleosomes Meiosis Histone Mutation Saccharomycetales biology.protein lcsh:Q Homologous recombination Gene Deletion Metabolic Networks and Pathways Research Article Protein Binding |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 5, p e0125965 (2015) |
| ISSN: | 1932-6203 |
| Popis: | In the meiotic prophase, programmed DNA double-strand breaks (DSB) are introduced along chromosomes to promote homolog pairing and recombination. Although meiotic DSBs usually occur in nucleosome-depleted, accessible regions of chromatin, their repair by homologous recombination takes place in a nucleosomal environment. Nucleosomes may represent an obstacle for the recombination machinery and their timely eviction and reincorporation into chromatin may influence the outcome of recombination, for instance by stabilizing recombination intermediates. Here we show in budding yeast that nucleosomes flanking a meiotic DSB are transiently lost during recombination, and that specific histone H3 chaperones, CAF-1 and Hir, are mobilized at meiotic DSBs. However, the absence of these chaperones has no effect on meiotic recombination, suggesting that timely histone reincorporation following their eviction has no influence on the recombination outcome, or that redundant pathways are activated. This study is the first example of the involvement of histone H3 chaperones at naturally occurring, developmentally programmed DNA double-strand breaks. |
| Databáze: | OpenAIRE |
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