Caspase/AIF/apoptosis pathway: a new target of puerarin for diabetes mellitus therapy
| Autor: | Biyun Gao, Dongmei Ye, Xiaohui Xu, Wenxia Chen, Tao Liang, Yanjun Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Blood Glucose medicine.medical_specialty Gene Expression Apoptosis Apoptosis-inducing factor Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Puerarin Internal medicine Type 2 diabetes mellitus Genetics medicine Animals Hypoglycemic Agents Insulin Molecular Biology Pancreas TUNEL assay Chemistry Apoptosis Inducing Factor Correction General Medicine Fasting Streptozotocin Isoflavones Lipids Caspase Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Mitochondrial respiratory chain Diabetes Mellitus Type 2 030220 oncology & carcinogenesis Caspases Original Article Biomarkers Lipoprotein medicine.drug Signal Transduction |
| Zdroj: | Molecular Biology Reports Mol Biol Rep |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Pancreatic β cell damage is one of the crucial factors responsible for the development of type 2 diabetes mellitus (T2DM). Previous studies have suggested that puerarin (PR) could regulate the activities of the mitochondrial respiratory chain complex in diabetic nephropathy (DN); however, whether PR can inhibit pancreatic β-cell apoptosis in T2DM remains to be elucidated. In the present study, T2DM mice induced by high-fat diet and streptozotocin (STZ) injection were used as a working model to investigate the mechanism of PR on pancreatic β cell apoptosis. The results showed that PR decreased the serum fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL) levels but significantly increased the fasting blood insulin (FINS) and high-density lipoprotein (HDL) levels. Furthermore, decreased caspase-3, 8, 9 and apoptosis-inducing factor (AIF) proteins in the pancreas were detected by Western blot analysis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) staining demonstrated that the pancreatic β cell apoptosis was inhibited by PR. Furthermore, PR improved the histopathological changes in pancreatic tissue in T2DM mice. Collectively, the data show that PR can protect the β cells from apoptotic death in a mouse model of T2DM through regulating the expression of apoptosis-related protein-AIF and caspase family proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink