Diagnostic benefits of adding EspC, EspF and Rv2348-B to the QuantiFERON Gold In-tube antigen combination
| Autor: | Morten Ruhwald, Maria Angeles Jimenez, Irene Latorre, Carmen Centeno, Cristina Prat, Adrián Sánchez-Montalvá, Esther García-García, Israel Molina-Pinargote, Javier Santiago, Carmen Martos, Fina Sabriá, Josep Domínguez, Adela Cantos, R. M. Guerola, Peter Andersen, Yoel Gonzalez-Diaz, J. Pilarte, Joan Pau Millet, M. L. De Souza-Galvão, Thomas Blauenfeldt, Irma Casas, Raquel Villar-Hernández, Beatriz Muriel-Moreno, J. Ruiz-Manzano |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male lcsh:Medicine Sensitivity and Specificity Article QuantiFERON 03 medical and health sciences 0302 clinical medicine Immune system Tuberculosis diagnosis Antigen Interferon Latent Tuberculosis medicine Tuberculosis Humans lcsh:Science Whole blood Antigens Bacterial Multidisciplinary Latent tuberculosis business.industry Tuberculin Test lcsh:R Diagnostic markers Mycobacterium tuberculosis Middle Aged medicine.disease 030104 developmental biology Early Diagnosis Spain Case-Control Studies Immunology lcsh:Q Female Interferon-gamma Release Tests business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| ISSN: | 2045-2322 |
| Popis: | Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to evaluate the addition of EspC, EspF and Rv2348-B to those present in the QuantiFERON-TB Gold In-Tube (QFN-G-IT). We included 115 subjects: 74 active TB patients, 17 LTBI individuals and 24 healthy controls. Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7.7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0.86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87.5% and 96.3%, respectively [AUC > 0.938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. Addition of these antigens could improve diagnosis in patients with impaired or immature immune response who are at high risk of developing TB. |
| Databáze: | OpenAIRE |
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