The dysregulation of metabolic pathways and induction of the pentose phosphate pathway in renal ischaemia–reperfusion injury
| Autor: | Alessandra Tammaro, Lotte Kors, Jaklien C. Leemans, Gwendoline J. D. Teske, Sandrine Florquin, Loes M. Butter, James D. Mills, Gerd Schmitz, Elena Rampanelli, Gerhard Liebisch, Joris J. T. H. Roelofs, Angelique M. L. Scantlebery |
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| Přispěvatelé: | Graduate School, Pathology, ACS - Diabetes & metabolism, ACS - Pulmonary hypertension & thrombosis, Amsterdam Gastroenterology Endocrinology Metabolism, Vascular Medicine |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine kidney medicine.medical_specialty pentose phosphate pathway Pentose phosphate pathway urologic and male genital diseases Fatty acid beta-oxidation Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine fatty acid beta-oxidation Internal medicine energy metabolism medicine Animals Glycolysis Original Paper Kidney urogenital system business.industry lipid accumulation Lipid metabolism Metabolism Acute Kidney Injury Peroxisome Lipid Metabolism Original Papers Mice Inbred C57BL Metabolic pathway 030104 developmental biology Endocrinology medicine.anatomical_structure Reperfusion Injury 030220 oncology & carcinogenesis fatty acid beta‐oxidation lipids (amino acids peptides and proteins) ischaemia–reperfusion injury business |
| Zdroj: | The Journal of Pathology Journal of pathology, 253(4), 404-414. John Wiley and Sons Ltd |
| ISSN: | 1096-9896 0022-3417 |
| Popis: | Lipid accumulation is associated with various forms of acute renal injury; however, the causative factors and pathways underpinning this lipid accumulation have not been thoroughly investigated. In this study, we performed lipidomic profiling of renal tissue following ischaemia–reperfusion injury (IRI). We identified a significant accumulation of cholesterol and specific phospholipids and sphingolipids in kidneys 24 h after IRI. In light of these findings, we hypothesised that pathways involved in lipid metabolism may also be altered. Through the analysis of published microarray data, generated from sham and ischaemic kidneys, we identified nephron‐specific metabolic pathways affected by IRI and validated these findings in ischaemic renal tissue. In silico analysis revealed the downregulation of several energy and lipid metabolism pathways, including mitochondrial fatty acid beta‐oxidation (FAO), peroxisomal lipid metabolism, fatty acid (FA) metabolism, and glycolysis. The pentose phosphate pathway (PPP), which is fuelled by glycolysis, was the only metabolic pathway that was upregulated 24 h following IRI. In this study, we describe the effect of renal IRI on metabolic pathways and how this contributes to lipid accumulation. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. |
| Databáze: | OpenAIRE |
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