IgE-Dependent Expression of Interleukin-5 mRNA and Protein in Human Lung: Modulation by Dexamethasone
| Autor: | Yihe Wang, Edward Dimitry, Jonathan S. Jaffe, Mark C. Glaum, Edward S. Schulman, Thomas J. Post, Donald G. Raible, David Gillespie |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_treatment
Immunology Gene Expression Inflammation Immunoglobulin E Dexamethasone Pathology and Forensic Medicine Gene expression medicine Humans Immunology and Allergy RNA Messenger Lung Interleukin 5 Messenger RNA biology Molecular biology Cytokine medicine.anatomical_structure biology.protein Interleukin-5 medicine.symptom medicine.drug |
| Zdroj: | Clinical Immunology and Immunopathology. 75:171-178 |
| ISSN: | 0090-1229 |
| Popis: | mRNA and protein expression of the Th2 cytokines IL-4 and IL-5 from human lung were examined during the first 4 hr following IgE-mediated triggering, a time representative of the evolving late-phase reaction (LPR). Lung explants were incubated for 16 hr at 37 degrees C in culture media alone or with added dexamethasone (10(-6) M), washed, and then challenged with buffer or anti-IgE (3 micrograms/ml). Using RNase protection assays, in 16/16 individual lungs IL-5 mRNA expression was observed at 4 hr following anti-IgE and at no points following buffer challenge. Fragments released 1129 +/- 499 ng of IL-5/g wet wt over a 24-hr period (mean +/- SEM, n = 5). Neither IL-4 transcripts nor protein were detected in any anti-IgE challenges. Both the IgE-mediated IL-5 mRNA and protein responses were below the limits of detection following dexamethasone preincubation, suggesting a mechanism for the potent inhibitory effects of these agents observed in the LPR. |
| Databáze: | OpenAIRE |
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