Decreased D2-40 and increased p16INK4A immunoreactivities correlate with higher grade of cervical intraepithelial neoplasia
| Autor: | Qizhi He, Yan Yang, Zhouping Lu, Zhenhua Lin, Hongxiu Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Histology government.form_of_government Down-Regulation Uterine Cervical Neoplasms Nerve Tissue Proteins Biology cervical intraepithelial neoplasia Cervical intraepithelial neoplasia Pathology and Forensic Medicine Basal (phylogenetics) Antibodies Monoclonal Murine-Derived D2-40 p16INK4A Antigens Neoplasm Predictive Value of Tests medicine Biomarkers Tumor lcsh:Pathology Humans Cyclin-Dependent Kinase Inhibitor p16 Chi-Square Distribution Research General Medicine medicine.disease Uterine Cervical Dysplasia female genital diseases and pregnancy complications Up-Regulation Lymphatic Endothelium immunohistochemistry government Immunohistochemistry Female Antigens neoplasm Neoplasm Grading lcsh:RB1-214 |
| Zdroj: | Diagnostic Pathology, Vol 6, Iss 1, p 59 (2011) Diagnostic Pathology |
| ISSN: | 1746-1596 |
| Popis: | Background D2-40 has been shown a selective marker for lymphatic endothelium, but also shown in the benign cervical basal cells. However, the application of D2-40 immunoreactivity in the cervical basal cells for identifying the grade of cervical intraepithelial neoplasia (CIN) has not been evaluated. Methods In this study, the immunoreactive patterns of D2-40, compared with p16INK4A, which is currently considered as the useful marker for cervical cancers and their precancerous diseases, were examined in total 125 cervical specimens including 32 of CIN1, 37 of CIN2, 35 of CIN3, and 21 of normal cervical tissue. D2-40 and p16INK4A immunoreactivities were scored semiquantitatively according to the intensity and/or extent of the staining. Results Diffuse D2-40 expression with moderate-to-strong intensity was seen in all the normal cervical epithelia (21/21, 100%) and similar pattern of D2-40 immunoreactivity with weak-to-strong intensity was observed in CIN1 (31/32, 97.2%). However, negative and/or focal D2-40 expression was found in CIN2 (negative: 20/37, 54.1%; focal: 16/37, 43.2%) and CIN3 (negative: 22/35, 62.8%; focal: 12/35, 34.3%). On the other hand, diffuse immunostaining for p16INK4A was shown in 37.5% of CIN1, 64.9% of CIN2, and 80.0% of CIN3. However, the immunoreactive pattern of D2-40 was not associated with the p16INK4A immunoreactivity. Conclusions Immunohistochemical analysis of D2-40 combined with p16INK4A may have a significant implication in clinical practice for better identifying the grade of cervical intraepithelial neoplasia, especially for distinguishing CIN1 from CIN2/3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink