Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells
| Autor: | Krittalak Chakrabandhu, Orawan Thongsum, Charoonroj Chotwiwatthanakun, Sébastien Huault, Pitchanee Jariyapong, Chidchanok Chawiwithaya, Wattana Weerachatyanukul, Anne-Odile Hueber, Khwanthana Grataitong |
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| Přispěvatelé: | Mahidol University [Bangkok], Navamindradhiraj University [Bangkok], Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Walailak University, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), HUEBER, Anne-Odile |
| Rok vydání: | 2021 |
| Předmět: |
viruses
Drug Compounding Recombinant Fusion Proteins [SDV]Life Sciences [q-bio] Science DNA Recombinant [SDV.CAN]Life Sciences [q-bio]/Cancer Sf9 02 engineering and technology Adenocarcinoma Biology Article Virus law.invention 03 medical and health sciences Drug Delivery Systems Plasmid [SDV.CAN] Life Sciences [q-bio]/Cancer Nanoscience and technology law Cell Line Tumor medicine Humans Gene silencing Nodaviridae Molecular Targeted Therapy Cancer 030304 developmental biology 0303 health sciences Multidisciplinary 021001 nanoscience & nanotechnology medicine.disease Virology Neoplasm Proteins ErbB Receptors [SDV] Life Sciences [q-bio] Capsid Drug Design Cancer cell Recombinant DNA Medicine Capsid Proteins Colorectal Neoplasms Peptides 0210 nano-technology |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-95891-x⟩ Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-95891-x⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-95891-x |
| Popis: | Recombinant MrNV capsid protein has been shown to effectively deliver plasmid DNA and dsRNA into Sf9 insect cells and shrimp tissues. To extend its application to cancer cell-targeting drug delivery, we created three different types of chimeric MrNV virus-like particles (VLPs) (R-MrNV, I-MrNV, and E-MrNV) that have specificity toward the epidermal growth factor receptor (EGFR), a cancer cell biomarker, by incorporating the EGFR-specific GE11 peptide at 3 different locations within the host cell recognition site of the capsid. All three chimeric MrNV-VLPs preserved the ability to form a mulberry-like VLP structure and to encapsulate EGFP DNA plasmid with an efficiency comparable to that previously reported for normal MrNV (N-MrNV). Compared to N-MrNV, the chimeric R-MrNV and E-MrNV carrying the exposed GE-11 peptide showed a significantly enhanced binding and internalization abilities that were specific towards EGFR expression in colorectal cancer cells (SW480). Specific targeting of chimeric MrNV to EGFR was proven by both EGFR silencing with siRNA vector and a competition with excess GE-11 peptide as well as the use of EGFR-negative colorectal cells (SW620) and breast cancer cells (MCF7). We demonstrated here that both chimeric R-MrNV and E-MrNV could be used to encapsulate cargo such as exogenous DNA and deliver it specifically to EGFR-positive cells. Our study presents the potential use of surface-modified VLPs of shrimp virus origin as nanocontainers for targeted cancer drug delivery. |
| Databáze: | OpenAIRE |
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