Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury
| Autor: | T. Giallongo, Anna Maria Di Giulio, Stephana Carelli, Claudio Gerace, Michele D. Basso, Alfredo Gorio, Anthea De Angelis |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty General Chemical Engineering Inflammation Regenerative medicine General Biochemistry Genetics and Molecular Biology Mice Neural Stem Cells medicine Animals Spinal cord injury Spinal Cord Injuries General Immunology and Microbiology business.industry General Neuroscience Tail vein Spinal cord medicine.disease Neural stem cell Surgery Transplantation Disease Models Animal medicine.anatomical_structure Medicine Stem cell medicine.symptom business Stem Cell Transplantation |
| Popis: | Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can be used both to investigate the biological responses to injury and to test potential therapies. Contusion or compression injury delivered to the surgically exposed spinal cord are the most widely used models of the pathology. In this report the experimental contusion is performed by using the Infinite Horizon (IH) Impactor device, which allows the creation of a reproducible injury animal model through definition of specific injury parameters. Stem cell transplantation is commonly considered a potentially useful strategy for curing this debilitating condition. Numerous studies have evaluated the effects of transplanting a variety of stem cells. Here we demonstrate an adapted method for spinal cord injury followed by tail vein injection of cells in CD1 mice. In short, we provide procedures for: i) cell labeling with a vital tracer, ii) pre-operative care of mice, iii) execution of a contusive spinal cord injury, and iv) intravenous administration of post mortem neural precursors. This contusion model can be utilized to evaluate the efficacy and safety of stem cell transplantation in a regenerative medicine approach. |
| Databáze: | OpenAIRE |
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