A ready-to-use dry powder formulation based on protamine nanocarriers for pulmonary drug delivery
| Autor: | Sandra Robla, Rubén Varela Calviño, Rita Ambrus, Noemi Csaba |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular, Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| Rok vydání: | 2023 |
| Předmět: |
Protamine nanocapsules
Anti-tuberculotic treatment eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107500RB-I00/ES/BIOMORPHIC PLATFORMS FOR THE PULMONARY DELIVERY OF NANOTHERAPEUTICS [info] Rifabutin Pharmaceutical Science 03.01. Általános orvostudomány Mannitol Pulmonary drug delivery |
| Zdroj: | European Journal of Pharmaceutical Sciences. 185:106442 |
| ISSN: | 0928-0987 |
| DOI: | 10.1016/j.ejps.2023.106442 |
| Popis: | The use of oral antibiotic therapy for the treatment of respiratory diseases as tuberculosis has promoted the appearance of side effects as well as resistance to these treatments. The low solubility, high metabolism, and degradation of drugs as rifabutin, have led to the use of combined and prolonged therapies, which difficult patient compliance. In this work, we develop inhalable formulations from biomaterials such as protamine to improve the therapeutic effect. Rifabutin-loaded protamine nanocapsules (NCs) were prepared by solvent displacement method and were physico-chemically characterized and evaluated for their dissolution, permeability, stability, cytotoxicity, hemocompatibility, internalization, and aerodynamic characteristics after a spray-drying procedure. Protamine NCs presented a size of around 200 nm, positive surface charge, and drug association up to 54%. They were stable as suspension under storage, as well as in biological media and as a dry powder after lyophilization in the presence of mannitol. Nanocapsules showed a good safety profile and cellular uptake with no tolerogenic effect on macrophages and showed good compatibility with red blood cells. Moreover, the aerodynamic evaluation showed a fine particle fraction deposition up to 30% and a mass median aerodynamic diameter of about 5 µm, suitable for the pulmonary delivery of therapeutics This research was funded by Ministerio de Ciencia e Innovacion RETOS - PID2019-107500RB-I00, Ministry of Human Capacities, Hungary grant TKP2021-EGA-32 and the Erasmus+ program of the European Union SI |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect