Discovering novel hydrolases from hot environments
| Autor: | Jennifer A. Littlechild, Xu Peng, Peter Menzel, Mark J. Young, Roland Wohlgemuth, Ilya V. Kublanov, Zalán Szabó, Bettina Siebers, Anne Gunn Rike, Kirk Matthew Schnorr, Daniela Monti, Georgios Skretas, Teunke van Rossum |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Thermophiles Hot Temperature Computer science Hydrolases 030106 microbiology Chemie Bioengineering Genomics Computational biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Enzyme Characterisation Bacterial Proteins Microbiologie Screening method Sequencing Bioprospecting High-Throughput Nucleotide Sequencing Enzyme characterization Enzyme screening Quorum sensing DNA Archaeal 030104 developmental biology Workflow Enrichment Metagenomics Epoxide Hydrolases Biocatalysis Metagenome Gene expression Thermoanaerobacterium Biotechnology |
| Zdroj: | Biotechnology Advances, 36(8), 2077-2100 Biotechnology advances 36 (2018): 2077–2100. doi:10.1016/j.biotechadv.2018.09.004 info:cnr-pdr/source/autori:Wohlgemuth R.; Littlechild J.; Monti D.; Schnorr K.; van Rossum T.; Siebers B.; Menzel P.; Kublanov I.V.; Rike A.G.; Skretas G.; Szabo Z.; Peng X.; Young M.J./titolo:Discovering novel hydrolases from hot environments/doi:10.1016%2Fj.biotechadv.2018.09.004/rivista:Biotechnology advances/anno:2018/pagina_da:2077/pagina_a:2100/intervallo_pagine:2077–2100/volume:36 Biotechnology Advances 36 (2018) 8 |
| ISSN: | 0734-9750 |
| DOI: | 10.1016/j.biotechadv.2018.09.004 |
| Popis: | Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes. |
| Databáze: | OpenAIRE |
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