Thermodynamics, Kinetics and Mechanisms of Noncompetitive Allosteric Inhibition of Chymotrypsin by Dihydrolipoic Acid-Coated Gold Nanoclusters
| Autor: | Wen-Qi Chen, Miao-Miao Yin, Peng-Jun Song, Xiao-Hang He, Feng-Lei Jiang, Yi Liu |
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| Rok vydání: | 2020 |
| Předmět: |
Allosteric regulation
Kinetics Metal Nanoparticles 02 engineering and technology 010402 general chemistry 01 natural sciences Nanomaterials Nanoclusters chemistry.chemical_compound Dihydrolipoic acid Electrochemistry Chymotrypsin General Materials Science Spectroscopy chemistry.chemical_classification Thioctic Acid biology fungi food and beverages Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Combinatorial chemistry 0104 chemical sciences Enzyme chemistry biology.protein Thermodynamics Gold 0210 nano-technology |
| Zdroj: | Langmuir. 36:6447-6457 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/acs.langmuir.0c00699 |
| Popis: | Enzymes are an important class of biomacromolecules which catalyze many metabolic processes in living systems. Nanomaterials can be synthesized with tailored sizes as well as desired surface modifications, thus acting as promising enzyme regulators. Fluorescent gold nanoclusters (AuNCs) are a representative class of ultrasmall nanoparticles (USNPs) with sizes of ∼2 nm, smaller than most of proteins including enzymes. In this work, we chose α-chymotrypsin (ChT) and AuNCs as the model system. Activity assays and inhibition kinetics studies showed that dihydrolipoic acid (DHLA)-coated AuNCs (DHLA-AuNCs) had a high inhibitory potency ( |
| Databáze: | OpenAIRE |
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