High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
| Autor: | Carlos Werning, Norberto Judewicz, Silvina Fenández Alvarez, Marcelo Criscuolo, Mariana Papouchado, Lino Barañao, Jorge Artuso, C H Carbonetto, Ignacio Berra, Claudio Santos, E. González, Luciana Muñoz, Carlos Munar, Aida Prync, Daniel Felipe Salamone, Nahuel Fernández, Marcelo Foti, Leonardo E. Bussmann, Susana Dabsys, Roberto Salaberry, Ignacio Mujica, Juan Zimmermann, Guillermo Berra, Carlos Melo |
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| Rok vydání: | 2006 |
| Předmět: |
Offspring
Cloning Organism Transgene Biotecnología Agropecuaria Bioengineering Biology Ética relacionada con Biotecnología Agrícola Applied Microbiology and Biotechnology law.invention Ciencias Biológicas Animals Genetically Modified law Animals Humans Cloning Genetics Human Growth Hormone Human growth hormone General Medicine Bioquímica y Biología Molecular Milk Proteins Recombinant Proteins Breed Transgenesis Biochemistry CIENCIAS AGRÍCOLAS Recombinant DNA Cattle CIENCIAS NATURALES Y EXACTAS Biotechnology Hormone |
| Zdroj: | Journal of Biotechnology. 124:469-472 |
| ISSN: | 0168-1656 |
| Popis: | Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Biosidus ; Argentina Fil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Santos, Claudio. Biosidus ; Argentina Fil: Bussmann, Leonardo Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Artuso, Jorge. Biosidus; Argentina Fil: Werning, Carlos. Biosidus; Argentina Fil: Prync, Aida. Biosidus; Argentina Fil: Carbonetto, Cesar. Biosidus; Argentina Fil: Dabsys, Susana. Biosidus; Argentina Fil: Munar, Carlos. Munar y Asociados; Argentina Fil: Salaberry R. Biosidus; Argentina Fil: Berra, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Berra, Ignacio. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Fernández, Nahuel. Biosidus ; Argentina Fil: Papouchado, Mariana. Biosidus; Argentina Fil: Foti, Marcelo. Biosidus; Argentina Fil: Judewicz, Norberto. Biosidus; Argentina Fil: Mujica, Ignacio. Munar y Asociados; Argentina Fil: Muñoz, Luciana. Biosidus; Argentina Fil: Fernandez Alvarez, Silvina. Biosidus; Argentina Fil: González, Eliseo. Centro de Diagnóstico Molecular; Argentina Fil: Zimmermann, Juan. Biosidus; Argentina Fil: Criscuolo, Marcelo. Biosidus; Argentina Fil: Melo, Carlos. Biosidus; Argentina |
| Databáze: | OpenAIRE |
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