Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats
| Autor: | Aleksa Čepić, Sanja Mihajlovic, Đorđe Miljković, Milica Lazarević, Miljana Momčilović, Bojan Jevtić, Katarina Veljović, Marija Mostarica Stojković, Suzana Stanisavljević, Nataša Golić, Neda Đedović, Svetlana Bojić |
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| Rok vydání: | 2019 |
| Předmět: |
Central Nervous System
0301 basic medicine Encephalomyelitis Autoimmune Experimental medicine.drug_class Firmicutes Encephalomyelitis Antibiotics lcsh:Medicine Administration Oral Autoimmunity Inflammation Gut flora Article 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals lcsh:Science Multidisciplinary biology lcsh:R Experimental autoimmune encephalomyelitis medicine.disease biology.organism_classification Anti-Bacterial Agents Gastrointestinal Microbiome Rats 3. Good health Disease Models Animal 030104 developmental biology Immunology Cytokines lcsh:Q medicine.symptom Dysbiosis 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system. |
| Databáze: | OpenAIRE |
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