| Autor: |
Oliver Teenan, Vishal Sahni, Robert B. Henderson, Bryan R. Conway, Carmel M. Moran, Jeremy Hughes, Laura Denby |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Teenan, O, Sahni, V, Henderson, R B, Conway, B R, Moran, C M, Hughes, J & Denby, L 2022, ' Sonoporation of human renal proximal tubular epithelial cells in vitro to enhance the liberation of intracellular miRNA biomarkers. ', Ultrasound in Medicine and Biology (UMB) . https://doi.org/10.1016/j.ultrasmedbio.2022.01.019 |
| DOI: |
10.1016/j.ultrasmedbio.2022.01.019 |
| Popis: |
Ultrasound has previously been demonstrated to non-invasively cause tissue disruption. Small animal studies have demonstrated that this effect can be enhanced by contrast microbubbles and has the potential to be clinically beneficial in techniques such as targeted drug delivery or enhancing liquid biopsies when a physical biopsy may be inappropriate. Cavitating microbubbles in close proximity to cells increases membrane permeability, allowing small intracellular molecules to leak into the extracellular space. This study sought to establish whether cavitating microbubbles could liberate cell-specific miRNAs, augmenting biomarker detection for non-invasive liquid biopsies. Insonating human polarized renal proximal tubular epithelial cells (RPTECs), in the presence of SonoVue microbubbles, revealed that cellular health could be maintained while achieving the release of miRNAs, miR-21, miR-30e, miR-192 and miR-194 (respectively, 10.9-fold, 7.17-fold, 5.95-fold and 5.36-fold). To examine the mechanism of release, RPTECs expressing enhanced green fluorescent protein were generated and the protein successfully liberated. Cell polarization, cellular phenotype and cell viability after sonoporation were measured by a number of techniques. Ultrastructural studies using electron microscopy showed gap-junction disruption and pore formation on cellular surfaces. These studies revealed that cell-specific miRNAs can be non-specifically liberated from RPTECs by sonoporation without a significant decrease in cell viability. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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