Cell-Membrane-Derived Nanoparticles with Notch-1 Suppressor Delivery Promote Hypoxic Cell-Cell Packing and Inhibit Angiogenesis Acting as a Two-Edged Sword
| Autor: | Gun-Il Im, Si Yeong Kim, Hak-Joon Sung, Songhyun Lee, Seyong Chung, Hye-Seon Kim, Se Won Yi, Mi-Lan Kang, Ji-Yun Ko, Dan Bi Park, Young Min Shin, Jeongeun Park, Sewoom Baek, Dahee Kim, Dae-Hyun Kim, Jung Bok Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Cell type
Materials science Angiogenesis Cell Survival Cell Palatine Tonsil Transplantation Heterologous Neovascularization Physiologic Cell membrane Mice Chondrocytes Neoplasms medicine Human Umbilical Vein Endothelial Cells Animals Humans General Materials Science Receptor Notch1 Drug Carriers Mechanical Engineering Mesenchymal stem cell Cell Membrane Cell Differentiation Mesenchymal Stem Cells Chondrogenesis Cadherins Cell biology medicine.anatomical_structure Mechanics of Materials Cancer cell Nanoparticles Intracellular Signal Transduction |
| Zdroj: | Advanced materials (Deerfield Beach, Fla.). 33(40) |
| ISSN: | 1521-4095 |
| Popis: | Cell-cell interactions regulate intracellular signaling via reciprocal contacts of cell membranes in tissue regeneration and cancer growth, indicating a critical need of membrane-derived tools in studying these processes. Hence, cell-membrane-derived nanoparticles (CMNPs) are produced using tonsil-derived mesenchymal stem cells (TMSCs) from children owing to their short doubling time. As target cell types, laryngeal cancer cells are compared to bone-marrow-derived MSCs (BMSCs) because of their cartilage damaging and chondrogenic characteristics, respectively. Treating spheroids of these cell types with CMNPs exacerbates interspheroid hypoxia with robust maintenance of the cell-cell interaction signature for 7 days. Both cell types prefer a hypoxic environment, as opposed to blood vessel formation that is absent in cartilage but is required for cancer growth. Hence, angiogenesis is inhibited by displaying the Notch-1 aptamer on CMNPs. Consequently, laryngeal cancer growth is suppressed efficiently in contrast to improved chondroprotection observed in a series of cell and animal experiments using a xenograft mouse model of laryngeal cancer. Altogether, CMNPs execute a two-edged sword function of inducing hypoxic cell-cell packing, followed by suppressing angiogenesis to promote laryngeal cancer death and chondrogenesis simultaneously. This study presents a previously unexplored therapeutic strategy for anti-cancer and chondroprotective treatment using CMNPs. |
| Databáze: | OpenAIRE |
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