β2-Containing Nicotinic Receptors Contribute to the Organization of Sleep and Regulate Putative Micro-Arousals in Mice
| Autor: | Joëlle Adrien, Pierre Escourrou, Régis Grailhe, Clément Léna, Daniela Popa, Jean-Pierre Changeux |
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| Přispěvatelé: | Récepteurs et Cognition (RC), Collège de France (CdF (institution))-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Neuropsychopharmacologie moléculaire, cellulaire et fonctionnelle, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neuropharmacologie, Université Paris-Sud - Paris 11 (UP11), Collège de France (CdF (institution))-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2004 |
| Předmět: |
Male
Hydrocortisone [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Receptors Nicotinic MESH: Mice Knockout MESH: Nicotine Nicotine Mice chemistry.chemical_compound 0302 clinical medicine Corticosterone MESH: Animals Nicotinic Agonists Mice Knockout 0303 health sciences Respiration musculoskeletal neural and ocular physiology General Neuroscience Electroencephalography MESH: Stress Psychological MESH: Sleep Deprivation MESH: Protein Subunits Sleep in non-human animals MESH: Hydrocortisone Plethysmography Nicotinic agonist MESH: Receptors Nicotinic Wakefulness medicine.symptom Arousal Psychology psychological phenomena and processes medicine.drug medicine.medical_specialty MESH: Sleep Rapid eye movement sleep Sleep REM Behavioral/Systems/Cognitive MESH: Electromyography Immobilization 03 medical and health sciences MESH: Mice Inbred C57BL Internal medicine MESH: Nicotinic Agonists MESH: Plethysmography MESH: Electroencephalography mental disorders medicine Animals MESH: Mice 030304 developmental biology MESH: Respiration Electromyography MESH: Arousal MESH: Sleep REM MESH: Immobilization MESH: Male Mice Inbred C57BL Protein Subunits MESH: Wakefulness Sleep deprivation Endocrinology chemistry Sleep Deprivation Sleep Stress Psychological 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, 2004, 24 (25), pp.5711-8. ⟨10.1523/JNEUROSCI.3882-03.2004⟩ Journal of Neuroscience, Society for Neuroscience, 2004, 24 (25), pp.5711-8. ⟨10.1523/JNEUROSCI.3882-03.2004⟩ |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.3882-03.2004 |
| Popis: | International audience; The cholinergic system is involved in arousal and in rapid eye movement sleep (REMS). To evaluate the contribution of nicotinic acetylcholine receptors (nAChRs) to these functions, we studied with polygraphic recordings the regulation of sleep in mice lacking the beta2 subunit gene of the nAChRs, a major component of high-affinity nicotine binding sites in the brain. Nicotine (1-2 mg/kg, i.p.) increased wakefulness in wild-type but not knock-out animals, indicating that beta2-containing nAChRs mediate the arousing properties of nicotine. Under normal conditions, the beta2-/- mice displayed the same amounts of waking, non-REM sleep (NREMS) and REMS as their wild-type counterparts. However, they exhibited longer REMS episodes and a reduced fragmentation of NREMS by events characterized notably by a transient drop in EEG power and frequently associated with EMG activation, tentatively referred to as micro-arousals. Respiration monitoring showed that these events were accompanied with, but not caused by, breathing irregularities. Sleep deprivation of beta2-/- mice resulted in a normal increase in REMS episode duration and NREMS delta power but yielded a reduction of the number of micro-arousals in NREMS. In contrast, in beta2-/- mice, a 1 hr immobilization stress failed to produce the normal rebound in REMS in the following 12 hr and, instead, was associated with increased NREMS fragmentation and sustained corticosterone levels. Our results show that the beta2-containing nAChRs contribute to the organization of sleep by regulating the transient phasic activity in NREMS, the REMS onset and duration, and the REMS-promoting effect of stress. |
| Databáze: | OpenAIRE |
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