Gluconeogenesis in goat hepatocytes is affected by calcium, ammonia and other key metabolites but not primarily through cytosolic redox state
| Autor: | R.J. Aiello, L.E. Armentano |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Physiology Metabolite chemistry.chemical_element Calcium Biology Biochemistry Ammonium Chloride chemistry.chemical_compound Cytosol Internal medicine medicine Animals Lactic Acid Molecular Biology chemistry.chemical_classification Ethanol Goats Gluconeogenesis Aminooxyacetic Acid General Medicine Metabolism Quinolinic Acid Quinolinic Acids Endocrinology medicine.anatomical_structure Liver chemistry Hepatocyte Lactates Propionate Ketone bodies Propionates Oxidation-Reduction |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 88:193-201 |
| ISSN: | 0305-0491 |
| DOI: | 10.1016/0305-0491(87)90100-3 |
| Popis: | 1. 1. Gluconeogenesis from propionate and lactate was studied in caprine hepatocytes. 2. 2. Reducing cytosol with additions of ETOH, ammonium, or lactate decreased [2- 14 C]propionate conversion to glucose. 3. 3. Calcium oxidized the cytosol and increased gluconeogenesis from propionate by 198% and from lactate by 220%. 4. 4. Cells isolated from lactating does and wethers differed quantitatively in propionate conversion to glucose and response to calcium. 5. 5. Acetoacetate decreased and 3-OH-butyrate slightly increased glucose production from propionate. 6. 6. Neither ketone body had any significant effect on gluconeogenesis from lactate. 7. 7. Results reported herein suggest gluconeogenesis from propionate is not limited by lack of cytosolic reducing equivalents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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