Perturbation of the metabolism of essential fatty acids by dietary partially hydrogenated vegetable oil
| Autor: | Ralph T. Holman, Eldon G. Hill, Larry D. Lawson, Susan B. Johnson, M. M. Mahfouz |
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| Rok vydání: | 1982 |
| Předmět: |
Fatty Acid Desaturases
Male Octadecenoic Acid Linoleic acid Oleic Acids Linoleic Acid chemistry.chemical_compound Oxidoreductase Animals Food science Phospholipids chemistry.chemical_classification Multidisciplinary Fatty Acids Essential Myocardium Fatty Acids Rats Inbred Strains Metabolism Dietary Fats Delta-6-desaturase Rats Linoleic Acids Liver chemistry Biochemistry Fatty Acids Unsaturated Microsome Corn oil Research Article Polyunsaturated fatty acid |
| Zdroj: | Proceedings of the National Academy of Sciences. 79:953-957 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Rats were fed purified diets containing (i) partially hydrogenated soybean oil as source of isomeric octadecenoic acids, (ii) hydrogenated coconut oil as source of saturated fatty acids, and (iii) a low level of corn oil as low-fat control. All diets contained 18% of the linoleate requirement. Rat liver and heart phospholipids were analyzed by gas/liquid chromatography for fatty acids, and liver, microsomes were assayed for desaturase (acyl-CoA, hydrogen-donor: oxidoreductase, EC 1.14.99.5) activities. Products of desaturation reactions measured analytically provided more information with greater statistical significance than did the enzymatic assays. Rats fed isomeric octadecenoic acids showed more severe essential fatty acid deficiency than did saturated-fat and control groups. The suppression of linoleate metabolites was largely due to decreased delta 5 and delta 6 desaturase activities. At several levels of linoleate, the deficiency was more severe at the higher level of isomeric octadecenoic acids. Increasing the intake of linoleate to 7.5% of calories did not suppress deposition of isomeric unsaturated acids in tissue lipids. |
| Databáze: | OpenAIRE |
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