Let-7 microRNA is a critical regulator in controlling the growth and function of silk gland in the silkworm
| Autor: | Chengyi Luo, Lili Xu, Wei Wang, Sanyuan Ma, Qingyou Xia, Quan Yin, Shiping Liu, Qian Pu, Xinran Wang, Xinyue Peng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Transgene
Silk Fluorescent Antibody Technique macromolecular substances Biology Models Biological Animals Genetically Modified 03 medical and health sciences Exocrine Glands 0302 clinical medicine Animals Endoreduplication Transgenes Molecular Biology 030304 developmental biology Gene Editing 0303 health sciences Gene knockdown Cell growth Gene Expression Profiling fungi technology industry and agriculture Nutrients Cell Biology Bombyx equipment and supplies Phenotype Pyruvate carboxylase Cell biology MicroRNAs SILK Gene Expression Regulation Gene Knockdown Techniques 030220 oncology & carcinogenesis Gene Targeting Nucleic Acid Conformation RNA Interference CRISPR-Cas Systems Energy Metabolism Transcriptome Function (biology) Signal Transduction Research Paper |
| Zdroj: | RNA Biol |
| ISSN: | 1555-8584 1547-6286 |
| DOI: | 10.1080/15476286.2020.1726128 |
| Popis: | The silk gland is characterized by high protein synthesis. However, the molecular mechanisms controlling silk gland growth and silk protein synthesis remain undetermined. Here we demonstrated that CRISPR/Cas9-based knockdown of let-7 or the whole cluster promoted endoreduplication and enlargement of the silk gland, accompanied by changing silk yield, whereas transgenic overexpression of let-7 led to atrophy and degeneration of the silk gland. Mechanistically, let-7 controls cell growth in the silk gland through coordinating nutrient metabolism processes and energy signalling pathways. Transgenic overexpression of pyruvate carboxylase, a novel target of let-7, resulted in enlargement of the silk glands, which is consistent with the abnormal phenotype of the let-7 knockdown. Overall, our data reveal a previously unknown miRNA-mediated regulation of silk gland growth and physiology and shed light on involvement of let-7 as a critical stabilizer and booster in carbohydrate metabolism, which may have important implications for understanding of the molecular mechanism and physiological function of specialized organs in other species. |
| Databáze: | OpenAIRE |
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