Novel osteogenic growth peptide C-terminal pentapeptide grafted poly(d,l-lactic acid) improves the proliferation and differentiation of osteoblasts: The potential bone regenerative biomaterial
| Autor: | Yueling Hong, Bin Wang, Zhen Zou, Liang Li, Chunjie Wen, Bingbing Zhang, Jun Zhang, Juan Xin, Rutao Hou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bone Regeneration Biocompatibility Cell Survival Polyesters Biocompatible Materials 02 engineering and technology Bone healing engineering.material Biochemistry Pentapeptide repeat Histones 03 medical and health sciences chemistry.chemical_compound Calcification Physiologic Structural Biology Amide Spectroscopy Fourier Transform Infrared Bone regeneration Molecular Biology Cells Cultured Cell Proliferation Osteoblasts Chemistry Photoelectron Spectroscopy Biomaterial Cell Differentiation General Medicine 021001 nanoscience & nanotechnology Lactic acid 030104 developmental biology Biophysics engineering Intercellular Signaling Peptides and Proteins Biopolymer 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | International Journal of Biological Macromolecules. 119:874-881 |
| ISSN: | 0141-8130 |
| Popis: | Poly(d,l-lactic acid) (PDLLA) is widely used for bone regenerative engineering, because of its proven biocompatibility and biodegradability. However, the major limitation of PDLLA is its cell recognition and low hydrophilicity. The objective of this study was to develop a novel bioactive poly(d,l-lactic acid) tethered with osteogenic growth peptide (OGP), which has been confirmed as one of the important growth factors related to bone repair/regeneration. The biomimetic material modification methods were utilized that maleic anhydride-modified poly(d,l-lactic acid) (MPLA) as raw material, the active C-terminal pentapeptide OGP(10-14) were covalently grafted onto the side chain of MPLA through amide reaction using 1‑ethyl‑3‑(3‑dimethyl aminopropyl) carbodiimide hydrochloride (EDC) and N‑hydroxysuccinimide (NHS) as the condensing agent to produce a new biopolymer (OGP(10-14)-MPLA). The OGP(10-14)-MPLA were further characterized with the Fourier transform infrared spectrometry, amino acid analyzer, elementary analysis, X-ray photoelectron spectroscopy. The results revealed that OGP(10-14) was successfully modified MPLA and its coupling efficiency was 12.40%. The data from both contact angle and water absorption showed the better hydrophilicity of OGP(10-14)-MPLA, compared with MPLA. Also, we found that OGP(10-14)-MPLA could improve the proliferation, differentiation, and mineralization of osteoblasts, indicating that the novel OGP(10-14)-MPLA has the better biocompatibility and is more osteoinductive. In conclusion, the OGP(10-14) modified MPLA have the potential for bone regenerative engineering. |
| Databáze: | OpenAIRE |
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