The proteoglycan repertoire of lymphoid cells
| Autor: | Svein Olav Kolset, Bodil Irene Fadnes, Lars Uhlin-Hansen, Anne Husebekk, Gunbjørg Svineng, Masaki Yanagishita, Øystein Rekdal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Glypican
Serglycin Cell Vesicular Transport Proteins Biochemistry Article Syndecan 1 chemistry.chemical_compound Cell Line Tumor medicine Humans Lymphocytes RNA Messenger RNA Neoplasm Molecular Biology VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical molecular biology: 711 biology Cell Biology Heparan sulfate VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical microbiology: 715 Cell biology Neoplasm Proteins carbohydrates (lipids) medicine.anatomical_structure Cell Transformation Neoplastic Proteoglycan chemistry Cell culture VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 Hematologic Neoplasms Immunology biology.protein Proteoglycans Syndecan-4 Heparitin Sulfate Syndecan CD8 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711 Lymphoid cells |
| Zdroj: | Glycoconjugate Journal |
| Popis: | Proteoglycans have been studied to a limited extent in lymphoid cells. In this study we have investigated the expression of proteoglycans in B-cells, CD4+ T-cells, CD8+ T-cells, natural killer cells, as well as in nine different cell lines established from patients with lymphoid malignancies. Serglycin was the major proteoglycan expressed at mRNA level by the primary lymphocytes. None of the syndecans or glycpicans was detected at mRNA level in the primary lymphocytes, except for syndecan-4 in CD4+ T-cells and CD8+ T-cells. All lymphoid cell lines expressed serglycin mRNA, as well as one or several members of the syndecan and glypican families. Further, increased synthesis of proteoglycans was found in the cell lines compared to the primary lymphocytes, as well as the presence of heparan sulfate on the cell surface of five of the cells lines. Western blot analysis showed a close correlation between serglycin mRNA level and expression of serglycin core protein. Our results show that serglycin is a major proteoglycan in all the normal lymphoid cells and that these cells carry little, or none, proteoglycans on the cell surface. Serglycin was also a major proteoglycan in the malignant lymphoid cells, but these also expressed one or more types of cell surface proteoglycans. Thus, malignant transformation of lymphoid cells may be followed by increased synthesis of proteoglycans and expression of cell surface proteoglycans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|