Chronic toxicity of chloroform to Japanese medaka fish
| Autor: | Florence J. Hoffmann, Marilyn J. Wolfe, Linda M. Brennan, Henry S. Gardner, Alan B. Rosencrance, Joseph R. Beaman, Margaret W. Toussaint |
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| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Antimetabolites Health Toxicology and Mutagenesis Metabolite Oryzias Hematocrit Biology chemistry.chemical_compound Water Supply Internal medicine medicine Animals Tissue Distribution Chronic toxicity Chloroform medicine.diagnostic_test Dose-Response Relationship Drug Liver Neoplasms Public Health Environmental and Occupational Health Japanese Medaka Dose–response relationship Endocrinology chemistry Bromodeoxyuridine Liver Bioaccumulation Histopathology Cell Division Disinfectants Research Article |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 0091-6765 |
| Popis: | Japanese medaka (Oryzias latipes) were continually exposed in a flow-through diluter system for 9 months to measured chloroform concentrations of 0.017, 0.151, or 1.463 mg/L. Parameters evaluated were hepatocarcinogenicity, hepatocellular proliferation, hematology, and intrahepatic chloroform concentration. Histopathology was evaluated at 6 and 9 months. Chloroform was not hepatocarcinogenic to the medaka at the concentrations tested. Chronic toxicity was evidenced at these time points by statistically significant ([alpha] = 0.05) levels of gallbladder lesions and bile duct abnormalities in medaka treated with 1.463 mg/L chloroform. We assessed hepatocellular proliferation by exposing test fish to 5-bromo-2'-deoxyuridine in the aquarium water for 72 hr after 4 and 20 days of chloroform exposure; we then quantified area-labeling indices of the livers using computer-assisted image analysis. We observed no treatment-related increases in cellular proliferation. We analyzed cells in circulating blood in medaka after 6 months of chloroform exposure. Hematocrit, leukocrit, cell viability, and cell counts of treated fish were not significantly different from those of control fish. Using gas chromatography (GC), we evaluated intrahepatic concentrations of chloroform in fish after 9 months of exposure. Livers from the 0.151 and 1.463 mg/L chloroform-treated fish had detectable amounts of chloroform, but these levels were always lower than the aquaria concentrations of chloroform. Thus, it appeared that chloroform did not bioaccumulate in the liver. Unidentified presumptive metabolite peaks were found in the GC tracings of these fish livers. |
| Databáze: | OpenAIRE |
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