MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE
| Autor: | N. V. Alpatieva, V. A. Gavrilova, E. B. Kuznetsova, A. G. Pinaev, I. N. Anisimova, V. T. Rozhkova, Yu. I. Karbitsina |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
pollen fertility restoration
sunflower molecular markers Physiology cms lines Plant Science Biology Biochemistry молекулярные маркеры линии гены цмс Genetics genes Molecular Biology Ecology Evolution Behavior and Systematics genetic collection Cytoplasmic male sterility Botany Sunflower Phenotype Cell biology генетическая коллекция генетика подсолнечник QK1-989 genetic восстановление фертильности пыльцы TP248.13-248.65 Biotechnology |
| Zdroj: | Труды по прикладной ботанике, генетике и селекции, Vol 177, Iss 2, Pp 99-107 (2018) |
| ISSN: | 2619-0982 2227-8834 |
| Popis: | Ninety five lines of sunflower genetic collection differing by their ability to suppress the CMS phenotype were molecularly marked with the use of 7 primer pairs. Using the STS marker orfH522, a sterile (PET1) cytoplasmon was identified in 79 lines, which confirmed indirectly the presence of fertility restoration genes in their genotypes. The majority of these lines also have a complex of molecular markers linked to the Rf1 gene. The HRG01, HRG02 and STS115 markers showed the best diagnostic value in revealing the Rf1 gene in the examined material. The data on allelic variation of the microsatellite loci ORS224, ORS511 and ORS799 were obtained for the first time. |
| Databáze: | OpenAIRE |
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