Modulation of the HSV-TK/ganciclovir bystander effect by n-butyrate in glioblastoma: correlation with gap-junction intercellular communication
| Autor: | Olivier Jolois, Matthew Nguyen, Vincent Bours, Brigitte Malgrange, Pierre A. Robe, Frédéric Princen, Marie-Paule Merville |
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| Rok vydání: | 2004 |
| Předmět: |
Ganciclovir
Cancer Research viruses Butyrate Cell Communication Biology Cell junction Thymidine Kinase Glioma Cell Line Tumor Bystander effect medicine Humans Simplexvirus Gap junction Gap Junctions Transfection Bystander Effect medicine.disease Virology Butyrates Trichostatin A Oncology Connexin 43 Cancer research Glioblastoma medicine.drug |
| Zdroj: | International journal of oncology. 25(1) |
| ISSN: | 1019-6439 |
| Popis: | The efficacy of HSV-TK/ganciclovir gene therapy largely relies on the bystander effect, i.e. the ability of transfected cells to kill the adjacent, untrasfected cells. This mechanism itself depends chiefly on the transfer via gap junctions of phosphorylated ganciclovir between cells, and is often deficient in glioblastomas. In this report, we demonstrate that n-butyrate markedly enhances the gap junction intercellular communication of GJIC-deficient glioma cells, and significantly increases the bystander effect in such cells. This effect of n-butyrate appears to be independent from its HDAC inhibitory effect, since trichostatin A does not reproduce it. |
| Databáze: | OpenAIRE |
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