Two independent grain-length mutants mapped to a single region on the long arm of chromosome 2 in rice
| Autor: | Zhiyong Zhang, Jingsheng Zheng, Fangyu Chen, Liangrong Jiang, Xiaolong Chen, Houcong Wang, Rongyu Huang, Yumin Huang, Zonglie Hong, Tiansheng Wang |
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| Rok vydání: | 2018 |
| Předmět: |
QTL mapping
0301 basic medicine Materials Science (miscellaneous) Mutant Population Quantitative trait locus Biology medicine.disease_cause Genetic analysis 03 medical and health sciences chemistry.chemical_compound Pollen Molecular marker medicine Oryza sativa L lcsh:Agriculture (General) education Gene marker-assisted breeding Genetics education.field_of_study food and beverages Chromosome grain length lcsh:S1-972 030104 developmental biology chemistry General Agricultural and Biological Sciences |
| Zdroj: | Bragantia, Volume: 77, Issue: 3, Pages: 452-465, Published: 02 AUG 2018 Bragantia, Issue: ahead, Published: 02 AUG 2018 Bragantia, Vol 77, Iss 3, Pp 452-465 (2018) Bragantia v.77 n.3 2018 Bragantia Instituto Agronômico de Campinas (IAC) instacron:IAC |
| ISSN: | 1678-4499 0006-8705 |
| Popis: | Grain shape in rice is a key determinant of grain appearance, yield and market value, and thus has been widely studied. Rice mutant lines with long-grain phenotypes were previously isolated from an M2 population derived from mutagenized mature pollen grains that were treated with gamma-ray irradiation for a cultivar Ma85. To understand the genetic basis underlying the long-grain trait, two mutant lines, JF171 and JF178, were crossed with the short-grain parents JF222 and Samba, generating three F2 populations. Molecular marker-based genetic analysis was employed to detect the major QTLs that affected grain length, grain width, length-to-width ratio and grain weight. Based on the data obtained for the three populations, a joint major QTL for grain length was mapped to a 4.7 Mb region between the SSR makers RM263 and RM318 on the long arm of chromosome 2. The results suggested that the two independent mutations contained in JF171 and JF178 are likely due to alterations in the same genetic region. Furthermore, we developed a BC2F2 population using JF178 and Samba and narrowed the region to 0.6 Mb. The results of the current study will be helpful to reveal the genetic basis of the above long-grain mutant lines. In general, our results provide solid genetic data to identify the unknown gene that affects grain length by map-based cloning and practice marker-assisted breeding for long-grain rice cultivars. |
| Databáze: | OpenAIRE |
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