Molecular cloning and antimicrobial activity of bombolitin, a component of bumblebee Bombus ignitus venom
| Autor: | Byung Rae Jin, Kwang Sik Lee, Seon-Woo Lee, Young Moo Choo, Hyung Joo Yoon, Hung Dae Sohn, Yeon Ho Je |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Gram-negative bacteria
Bombus ignitus Physiology Molecular Sequence Data Peptide Venom Molecular cloning complex mixtures Biochemistry Melittin Microbiology chemistry.chemical_compound Anti-Infective Agents Animals Amino Acid Sequence Cloning Molecular Molecular Biology Bumblebee chemistry.chemical_classification Base Sequence biology biology.organism_classification Bee Venoms chemistry Peptides Antibacterial activity |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 156:168-173 |
| ISSN: | 1096-4959 |
| Popis: | Bombolitin is the most abundant component of bumblebee venom and shares structural and biological properties with melittin, a component of honeybee venom. Here, we describe the molecular cloning and antimicrobial activity of bombolitin isolated from the venom of the bumblebee Bombus ignitus. The B. ignitus bombolitin gene consists of 2 exons encoding 56-amino acid residues. The bombolitin isolated from B. ignitus venom is a 1.99 kDa mature peptide with 18-amino acid residues, and it is created by the cleavage of the probombolitin domain between Ala38 and Leu39. B. ignitus bombolitin exhibits venom gland-specific expression. We also investigated the antimicrobial properties of B. ignitus bombolitin against bacteria and fungi. The B. ignitus bombolitin showed high antibacterial activity against two Gram-positive and two Gram-negative bacteria. In addition, the B. ignitus bombolitin displayed antifungal activity against the plant pathogenic fungi Fulvia fulva and Alternaria radicina. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect