Time-dependent urinary bladder remodeling in the streptozotocin-induces diabetic rat model
| Autor: | John Anthony Bauer, D. A. Pitre, L. J. Wallace, T. Ma |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Endocrinology Diabetes and Metabolism Urinary system Urinary Bladder H&E stain urologic and male genital diseases Diabetes Mellitus Experimental Muscle hypertrophy Rats Sprague-Dawley Masson's trichrome stain Endocrinology Internal Medicine Animals Medicine Urothelium Total Tissue Urinary bladder business.industry Reproducibility of Results Muscle Smooth General Medicine Streptozotocin female genital diseases and pregnancy complications Rats medicine.anatomical_structure Collagen business medicine.drug |
| Zdroj: | Acta Diabetologica. 39:23-27 |
| ISSN: | 1432-5233 0940-5429 |
| DOI: | 10.1007/s005920200008 |
| Popis: | Urinary bladder dysfunction and remodeling are well-recognized phenomena in diabetes but detailed assessments of tissue morphological changes have not been conducted. We studied time-dependent morphological changes in bladders from diabetic rats (streptozotocin model) and evaluated the usefulness of automated digital imaging technology as an unbiased, reproducible, and convenient method for the bladder morphometric analysis. Urinary bladders were isolated from diabetic (3 days, 2 weeks or 5 weeks after single injection of streptozotocin, 65 mg/kg) or control rats (0 or 5 weeks) and were processed for histochemical evaluations (hematoxylin/eosin and Mason's trichrome staining). Digital image analysis was used to quantify equatorial cross-sectional areas of bladder tissue and lumen, as well as relative prevalence of the three primary tissue components viz. smooth muscle, urothelium, and extracellular matrix. Digital imaging and color segmentation provided reliable and unbiased evaluations of the bladder tissue sections. Progressive increases in total bladder tissue and lumen area were observed in the diabetic animals relative to controls (p |
| Databáze: | OpenAIRE |
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