Fluorescent, Bioluminescent, and Optogenetic Approaches to Study Excitable Physiology in the Single Cardiomyocyte
| Autor: | Paul Robinson, Matthew J. Daniels, Connor N Broyles |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Computer science Physiology cardiomyocyte Context (language use) Review Optogenetics 03 medical and health sciences fluorescent protein Bioluminescence Fluorescent protein optogenetics chemical dye lcsh:QH301-705.5 Drug toxicity excitable cell physiology calcium Drug discovery General Medicine bioluminescence adult ventricular cardiomyocyte Disease modelling 030104 developmental biology lcsh:Biology (General) Optical control voltage FRET iPS-cardiomyocyte |
| Zdroj: | Cells, Vol 7, Iss 6, p 51 (2018) Broyles, C, Robinson, P & Daniels, M 2018, ' Fluorescent, Bioluminescent, and Optogenetic Approaches to Study Excitable Physiology in the Single Cardiomyocyte ', Cells, vol. 7, no. 6, pp. 51 . https://doi.org/10.3390/cells7060051 Cells |
| ISSN: | 2073-4409 |
| Popis: | This review briefly summarizes the single cell application of classical chemical dyes used to visualize cardiomyocyte physiology and their undesirable toxicities which have the potential to confound experimental observations. We will discuss, in detail, the more recent iterative development of fluorescent and bioluminescent protein-based indicators and their emerging application to cardiomyocytes. We will discuss the integration of optical control strategies (optogenetics) to augment the standard imaging approach. This will be done in the context of potential applications, and barriers, of these technologies to disease modelling, drug toxicity, and drug discovery efforts at the single-cell scale. |
| Databáze: | OpenAIRE |
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