Allergens involved in the cross-reactivity of Aedes aegypti with other arthropods
| Autor: | Leonardo Puerta, José Fernando Cantillo, José Luis Subiza, Enrique Fernández-Caldas, Luis Caraballo, Sylvie Lafosse-Marin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Adult Male Allergy animal structures Adolescent Immunology Magnesium ion binding Aedes aegypti Tropomyosin Cross Reactions Immunoglobulin E medicine.disease_cause Cross-reactivity Microbiology Arthropod Proteins 03 medical and health sciences Young Adult 0302 clinical medicine Blood serum biology.animal Mite medicine Immunology and Allergy Animals Humans Child Arthropods Cockroach biology fungi Infant Allergens Middle Aged Peptidylprolyl Isomerase biology.organism_classification medicine.disease Rhinitis Allergic Asthma Recombinant Proteins 030104 developmental biology 030228 respiratory system Child Preschool biology.protein Female |
| Zdroj: | Annals of allergy, asthmaimmunology : official publication of the American College of Allergy, Asthma,Immunology. 118(6) |
| ISSN: | 1534-4436 |
| Popis: | Background Cross-reactivity between Aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. Objective To evaluate the cross-reactivity between A aegypti and other arthropods. Methods Thirty-four serum samples from patients with asthma and/or allergic rhinitis were selected, and specific IgE to A aegypti , Dermatophagoides pteronyssinus , Dermatophagoides farinae , Blomia tropicalis , Periplaneta americana . and Litopenaeus vannamei was measured by enzyme-linked immunosorbent assay. Cross-reactivity was investigated using pooled serum samples from allergic patients, allergenic extracts, and the recombinant tropomyosins (Aed a 10.0201, Der p 10, Blo t 10, Lit v 1, and Per a 7). Four IgE reactive bands were further characterized by matrix-assisted laser desorption/ionization tandem time of flight. Results Frequency of positive IgE reactivity was 82.35% to at least one mite species, 64.7% to A aegypti , 29.4% to P americana , and 23.5% to L vannamei . The highest IgE cross-reactivity was seen between A aegypti and D pteronyssinus (96.6%) followed by L vannamei (95.4%), B tropicalis (84.4%), and P americana (75.4%). Recombinant tropomyosins from mites, cockroach, or shrimp inhibited the IgE reactivity to the mosquito at a lower extent than the extracts from these arthropods. Several bands of A aegypti cross-reacted with arthropod extracts, and 4 of them were identified as odorant binding protein, mitochondrial cytochrome C, peptidyl-prolyl cis-trans isomerase, and protein with hypothetical magnesium ion binding function. Conclusion We identified 4 novel cross-reactive allergens in A aegypti allergenic extract. These molecules could influence the manifestation of allergy to environmental allergens in the tropics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|