Assessment of reflectance confocal microscopy for non-invasive selection of optimal ovarian cortex fragments for autotransplantation

Autor: M. Peppelman, M.j. Schleedoorn, Norah M. van Mello, Jana Liebenthron, Kathrin Fleischer, Willianne L.D.M. Nelen, Ron Peek, Piet E.J. van Erp, Hans van der Ven, Catharina C. M. Beerendonk, Didi D.M. Braat
Přispěvatelé: Obstetrics and gynaecology, Amsterdam Reproduction & Development (AR&D)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Adult
Blood Glucose
Neutral red
Ovarian Cortex
Adolescent
Transplantation
Autologous
Cryopreservation
Andrology
Tissue Culture Techniques
03 medical and health sciences
chemistry.chemical_compound
Follicle
Young Adult
All institutes and research themes of the Radboud University Medical Center
0302 clinical medicine
Ovarian Follicle
Animals
Humans
Ovarian tissue cryopreservation
Child
030219 obstetrics & reproductive medicine
Microscopy
Confocal
Ovary
Obstetrics and Gynecology
Fertility Preservation
Histology
Equipment Design
Women's cancers Radboud Institute for Health Sciences [Radboudumc 17]
Staining
030104 developmental biology
Reproductive Medicine
chemistry
Neutral Red
Child
Preschool
Oocytes
Cattle
Female
Infertility
Female
Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5]
Ex vivo
Developmental Biology
Zdroj: Schleedoorn, M J, Peppelman, M, van Erp, P E J, Beerendonk, C C M, Nelen, W L D M, Braat, D D M, van Mello, N M, Liebenthron, J, van der Ven, H, Fleischer, K & Peek, R 2019, ' Assessment of reflectance confocal microscopy for non-invasive selection of optimal ovarian cortex fragments for autotransplantation ', Reproductive BioMedicine Online, vol. 38, no. 6, pp. 999-1009 . https://doi.org/10.1016/j.rbmo.2018.12.024
Reproductive Biomedicine Online, 38, 999-1009
Reproductive Biomedicine Online, 38, 6, pp. 999-1009
Reproductive BioMedicine Online, 38(6), 999-1009. Elsevier
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2018.12.024
Popis: Research question Can reflectance confocal microscopy (RCM) be used to determine follicle density in human ovarian cortex fragments that are intended for fertility restoration? Design RCM was used on living cortex tissue fragments derived from five bovine ovaries and 13 human ovaries. All tissue fragments were cryopreserved and thawed before RCM analysis. Follicle numbers and distribution were determined by RCM and histology. Before and after RCM, general tissue viability and follicle integrity were assessed by a glucose uptake assay and neutral red staining, respectively. Results RCM can detect all stages of follicle development in living ovarian tissue to a maximum depth of 250 µm. In bovine tissue, all follicles were located within this 0–250 µm range. In human ovarian tissue, follicles were also present below the 250 µm RCM threshold, implying that only a percentage of the total number of follicles could be detected with RCM. The percentage of follicles detected by RCM appeared to be age dependent. The RCM procedure did not affect the glucose uptake by the tissue, whereas neutral red staining indicated a high level of follicle survival. Conclusion In this proof of concept study, we have shown that RCM is a promising technique to determine the density of follicles ex vivo in living human ovarian cortex fragments, apparently without compromising the vitality of the tissue. Safety studies and further optimization of the RCM technique with a focus on increasing the penetration depth are required before clinical use of RCM.
Databáze: OpenAIRE
Externí odkaz: