Status of kinases in Epstein-Barr virus and Helicobacter pylori Coinfection in gastric Cancer cells

Autor: Debi Chatterji, Hem Chandra Jha, Charu Sonkar, Tarun Verma, Ajay Jain
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Epstein-Barr Virus Infections
Herpesvirus 4
Human
Cancer Research
Biology
medicine.disease_cause
lcsh:RC254-282
Helicobacter Infections
03 medical and health sciences
0302 clinical medicine
FYN
Stomach Neoplasms
Cell Line
Tumor
Genetics
medicine
Humans
Interleukin-2-inducible T-cell kinase
Cell Proliferation
Helicobacter pylori
Tyrosine-protein kinase Fyn
Coinfection
Kinase
Gene Expression Profiling
Phosphotransferases
Epstein Barr virus
Adenocarcinoma gastric cell
biology.organism_classification
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Epstein–Barr virus
Molecular biology
Gene Expression Regulation
Neoplastic
Gene expression profiling
030104 developmental biology
Oncology
Gastric Mucosa
Tyrosine kinase 2
030220 oncology & carcinogenesis
Cancer cell
Tyrosine-Protein Kinase Fyn
Gastric cancer
Research Article
Zdroj: BMC Cancer, Vol 20, Iss 1, Pp 1-14 (2020)
BMC Cancer
ISSN: 1471-2407
Popis: Background Helicobacter pylori (H. pylori) and Epstein - Barr virus (EBV) plays a significant role in aggressive gastric cancer (GC). The investigation of genes associated with these pathogens and host kinases may be essential to understand the early and dynamic progression of GC. Aim The study aimed to demonstrate the coinfection of EBV and H. pylori in the AGS cells through morphological changes, expression of the kinase and the probable apoptotic pathways. Methods Genomic DNA isolation of H. pylori and its characterization from clinical samples were performed. RT-qPCR of kinases was applied to scrutinize the gene expression of kinases in co-infected GC in a direct and indirect (separated through insert size 0.45 μm) H. pylori infection set up. Morphological changes in co-infected GC were quantified by measuring the tapering ends of gastric epithelial cells. Gene expression profiling of apoptotic genes was assessed through RT-qPCR. Results An interleukin-2-inducible T-cell kinase (ITK) showed significant upregulation with indirect H. pylori infection. Moreover, Ephrin type-B receptor six precursors (EPHB6) and Tyrosine-protein kinase Fyn (FYN) showed significant upregulation with direct coinfection. The tapering ends in AGS cells were found to be extended after 12 h. A total of 24 kinase genes were selected, out of which EPHB6, ITK, FYN, and TYK2 showed high expression as early as 12 h. These kinases may lead to rapid morphological changes in co-infected gastric cells. Likewise, apoptotic gene expression such as APAF-1 and Bcl2 family genes such as BAD, BID, BIK, BIM, BAX, AND BAK were significantly down-regulated in co-infected AGS cells. Conclusion All the experiments were performed with novel isolates of H. pylori isolated from central India, for the functional assessment of GC. The effect of coinfection with EBV was more profoundly observed on morphological changes in AGS cells at 12 h as quantified by measuring the tapering of ends. This study also identifies the kinase and apoptotic genes modulated in co-infected cells, through direct and indirect approaches. We report that ITK, EPHB6, TYK2, FYN kinase are enhanced, whereas apoptotic genes such as APAF-1, BIK, FASL, BAX are significantly down-regulated in AGS cells coinfected with EBV and H. pylori.
Databáze: OpenAIRE
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