On-chip determination of C-reactive protein using magnetic particles in continuous flow
| Autor: | Sally A. Peyman, John Greenman, Mark D. Tarn, Nicole Pamme, Chayakom Phurimsak |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Detection limit
Immunoassay Chromatography Chemistry Continuous flow Magnetic Phenomena Laminar flow Magnetic particle inspection Equipment Design Microfluidic Analytical Techniques Analytical Chemistry Magnetics C-Reactive Protein Limit of Detection Magnet Reagent Magnets Magnetic nanoparticles Humans Inflammatory biomarker |
| Zdroj: | Analytical chemistry. 86(21) |
| ISSN: | 1520-6882 |
| Popis: | We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the inflammatory biomarker, C-reactive protein (CRP). The two-step sandwich immunoassay was accomplished in less than 60 s, a vast improvement on the 80-300 min time frame required for enzyme-linked immunosorbent assays (ELISA) and the 50 min necessary for off-chip magnetic particle-based assays. The combination of continuous flow and a stationary magnet enables a degree of autonomy in the system, while a detection limit of 0.87 μg mL(-1) makes it suitable for the determination of CRP concentrations in clinical diagnostics. Its applicability was further proven by assaying real human serum samples and comparing those results to values obtained using standard ELISA tests. |
| Databáze: | OpenAIRE |
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