A comparison of the effectiveness of QuEChERS, FaPEx and a modified QuEChERS method on the determination of organochlorine pesticides in ginseng
| Autor: | Hyo-Sub Lee, Jin Hyo Kim, Geun-Hyoung Choi, Pennante Bruce-Vanderpuije, Byung-Seok Kim, David Megson, Song-Hee Ryu, Sang-Won Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Insecticides
010501 environmental sciences 01 natural sciences Ginseng chemistry.chemical_compound Limit of Detection Agricultural Soil Science Hydrocarbons Chlorinated Extraction Techniques Multidisciplinary Sulfates Organic Compounds Supercritical fluid extraction Agriculture Chemistry Physical Sciences Medicine Agrochemicals Endosulfan Research Article Science Panax Soil Science Research and Analysis Methods Quechers Gas Chromatography-Mass Spectrometry Ddt Acetones Hexanes Dichlorodiphenyldichloroethane Pesticides Supercritical Fluid Extraction 0105 earth and related environmental sciences Detection limit Chromatography Organic Chemistry 010401 analytical chemistry Extraction (chemistry) Pesticide Residues Chemical Compounds Biology and Life Sciences Pesticide Hydrocarbons 0104 chemical sciences chemistry Earth Sciences Salts Pest Control Gas chromatography–mass spectrometry |
| Zdroj: | PLoS ONE, Vol 16, Iss 1, p e0246108 (2021) PLoS ONE |
| Popis: | This study provides a review of methods used in the determination of organochlorine pesticides (OCPs) in ginseng and compares the effectiveness of three extraction methods (Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS), a modified QuEChERS and a Fast Pesticide Extraction (FaPEx)) in the analyses of 20 OCPs in ginseng root samples. For each method, sample mass, solvent volume and sorbent mass were varied to identify the optimum combination to effectively isolate analytes of interest from the complex sample matrix. Extracts were analyzed using the gas chromatography-μ-electron capture detector (GC-μ-ECD), and confirmatory analyses performed by gas chromatography-tandem-mass spectrometry (GC-MS/MS). Eighteen out of 20 OCPs spiked onto in-house prepared ginseng samples produced acceptable recoveries (51–156%) when extracted using QuEChERS and FaPEx. All 20 analytes, including dichlorodiphenyldichloroethane (p, p’- DDD) and dichlorodiphenyltrichloroethane (o, p’-DDT), produced acceptable recoveries (51–129%) with the use of a modified QuEChERS method. The applicability of the modified QuEChERS method was demonstrated through the analysis of ginseng samples grown in endosulfan-treated soil. The samples were analyzed by both GC-μ-ECD and GC-MS/MS with no significant difference identified in the results of each analytical method. This study highlights the applicability of the modified QuEChERS method, in combination with GC- μ-ECD, to determine organochlorine pesticides in ginseng. This may be especially useful for laboratories in developing countries and less advanced institutions without access to MS/MS instrumentation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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