Identification of autoreactive B cells with labeled nucleosomes
| Autor: | Cécile Seifert, A. Guffroy, Thierry Martin, Anne-M Knapp, Anne-S Korganow, Jean-L Pasquali, Jean-D Fauny, Pauline Soulas-Sprauel, Vincent Gies, Alain Wagner, Hélène Dumortier |
|---|---|
| Přispěvatelé: | Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Conception et application de molécules bioactives (CAMB), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Systèmes Fonctionnels, Centre National de la Recherche Scientifique (CNRS), Immunopathologie et chimie thérapeutique (ICT), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Science Transgene Autoimmunity [SDV.BC]Life Sciences [q-bio]/Cellular Biology medicine.disease_cause Autoantigens Article Cell Line Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Sciences du Vivant [q-bio]/Biologie cellulaire medicine Animals Humans Lupus Erythematosus Systemic B cell Autoantibodies B-Lymphocytes Multidisciplinary Lupus erythematosus Staining and Labeling biology medicine.diagnostic_test Autoantibody Flow Cytometry medicine.disease Molecular biology Nucleosomes 3. Good health Cell biology 030104 developmental biology Histone medicine.anatomical_structure Cell culture biology.protein Medicine Female Biomarkers 030215 immunology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/s41598-017-00664-0⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-00664-0 |
| Popis: | The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|