Comparison of enantioselective HPLC separation of structurally diverse compounds on chiral stationary phases with different teicoplanin coverage and distinct linkage chemistry
| Autor: | Eva Tesarova, Simona Srkalová, Marie Honetschlägerová-Vadinská, Zuzana Bosáková, Pavel Coufal |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Time Factors Chromatography Molecular Structure Teicoplanin Hydrophilic interaction chromatography Stereoisomerism Filtration and Separation Amino Alcohols High-performance liquid chromatography Analytical Chemistry Amino acid chemistry.chemical_compound chemistry Phase (matter) medicine Methanol Amino Acids Propionates Enantiomer Aliphatic compound Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Separation Science. 32:1704-1711 |
| ISSN: | 1615-9306 |
| Popis: | Enantioseparation performance of two teicoplanin-based chiral stationary phases, Chirobiotic T and Chirobiotic T2, with different teicoplanin coverage and distinct linkage chemistry was compared. Three structurally diverse groups of analytes, amino alcohols (beta-blockers), chlorophenoxypropionic acids, and branched-chain amino acids, in various mobile phase compositions/separation modes were examined. The amino alcohols showed better enantioresolution on Chirobiotic T2 in reverse-phase, hydrophilic interaction chromatography, and polar-ionic mode separation systems. The best results with these analytes were obtained in the polar-ionic mobile phase. In contrast, the chlorophenoxypropionic acids and the branched-chain amino acids yielded an improved enantioresolution on the chiral stationary phase with lower amount of teicoplanin in the hydrophilic interaction chromatography system. The comparison of separation of the chlorophenoxypropionic acids enantiomers in the reverse-phase and hydrophilic interaction chromatography environments showed completely opposite results. While better enantioresolution of chlorophenoxypropionic acids was achieved on Chirobiotic T2 in mobile phases with low methanol content, high methanol concentration was needed to reach baseline enantioseparation on Chirobiotic T. |
| Databáze: | OpenAIRE |
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