A systems approach to analyze transcription factors in mammalian cells
| Autor: | Erikjan Rijkers, Eric Soler, Jeroen Demmers, Frank Grosveld, Supat Thongjuea, Ernie de Boer, Charlotte Andrieu-Soler, Wilfred F. J. van IJcken, Jan Christian Bryne |
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| Přispěvatelé: | Cell biology, Biochemistry |
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Chromatin Immunoprecipitation Binding Sites Embryo Nonmammalian Hematopoietic cell Systems Biology Cell Differentiation Computational biology Biology Embryo Mammalian Pipeline (software) General Biochemistry Genetics and Molecular Biology DNA sequencing Mice Haematopoiesis Multicellular organism Gene Expression Regulation Animals Molecular Biology Gene Transcription factor Zebrafish Function (biology) Transcription Factors |
| Zdroj: | Methods-A Companion to Methods in Enzymology, 53(2), 151-162. Academic Press |
| ISSN: | 1095-9130 1046-2023 |
| Popis: | Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation. (C) 2010 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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