An Adolescent Porcine Model of Voluntary Alcohol Consumption Exhibits Binge Drinking and Motor Deficits in a Two Bottle Choice Test
Autor: | Jesse R. Schank, Soo K. Shin, Sydney E Sneed, Matthew G Solomon, Franklin D. West, Sadie E. Nennig, Savannah R Cheek, Holly A. Kinder |
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Rok vydání: | 2020 |
Předmět: |
Swine
030508 substance abuse Physiology Binge drinking Alcohol Binge Drinking 03 medical and health sciences chemistry.chemical_compound Saccharin 0302 clinical medicine Alcohol intoxication medicine Animals Ethanol metabolism Ethanol business.industry General Medicine medicine.disease Gait chemistry Gait analysis Models Animal Blood Alcohol Content 0305 other medical science business Alcoholic Intoxication 030217 neurology & neurosurgery |
Zdroj: | Alcohol and Alcoholism. 56:266-274 |
ISSN: | 1464-3502 0735-0414 |
Popis: | Aims Alcohol is the most commonly abused substance leading to significant economic and medical burdens. Pigs are an attractive model for studying alcohol abuse disorder due to the comparable alcohol metabolism and consumption behavior, which are in stark contrast to rodent models. This study investigates the usage of a porcine model for voluntary binge drinking (BD) and a detailed analysis of gait changes due to motor function deficits during alcohol intoxication. Methods Adolescent pigs were trained to drink increasing concentration (0–8%) of alcohol mixed in a 0.2% saccharin solution for 1 h in a two bottle choice test for 2 weeks. The training period was followed by a 3-week alcohol testing period, where animals were given free access to 8% alcohol in 0.2% saccharin solution and 0.2% saccharin water solution. Blood alcohol levels were tested and gait analysis was performed pre-alcohol consumption, last day of training, and Day 5 of each testing period. Results Pigs voluntarily consumed alcohol to intoxication at all timepoints with blood alcohol concentration (BAL) ≥80 mg/dl. Spatiotemporal gait parameters including velocity, cadence, cycle time, swing time, stance time, step time, and stride length were perturbed as a result of intoxication. The stratification of the gait data based on BAL revealed that the gait parameters were affected in a dose-dependent manner. Conclusion This novel adolescent BD porcine model with inherent anatomical and physiological similarities to humans display similar consumption and intoxication behavior that is likely to yield results that are translatable to human patients. |
Databáze: | OpenAIRE |
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